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Isolation and characterisation of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence

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Summary

The blackleg pathogen Erwinia carotovora subsp. atroseptica (Eca) causes an economically important disease of potatoes. We selected a genetically amenable Eca strain for the genetic analysis of virulence. Tn5 mutagenesis was used to generate nine mutants which exhibited reduced virulence (Rvi-) of strain SCRI1043. Following physiological characterisation, mutants were divided into three classes: (1) auxotrophs; (2) extracellular enzyme mutants; and (3) a growth rate mutant. The isolation of these Rvi- mutants has allowed us to consider some factors that affect Eca virulence.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Warwick, CV4 7AL, Coventry, England

    Jay C. D. Hinton, Julie M. Sidebotham & George P. C. Salmond

  2. Scottish Crop Research Institute, Invergowrie, DD2 5DA, Dundee, Scotland

    Lizbeth J. Hyman & Michel C. M. Pérombelon

Authors
  1. Jay C. D. Hinton
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  2. Julie M. Sidebotham
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  3. Lizbeth J. Hyman
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  4. Michel C. M. Pérombelon
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  5. George P. C. Salmond
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Communicated by H. Hennecke

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Hinton, J.C.D., Sidebotham, J.M., Hyman, L.J. et al. Isolation and characterisation of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence. Mol Gen Genet 217, 141–148 (1989). https://doi.org/10.1007/BF00330953

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  • DOI: https://doi.org/10.1007/BF00330953

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