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Ultrastruktur glycerinextrahierter Dünndarmmuskelzellen der Ratte vor und nach Kontraktion

Ultrastructure of glycerinated small intestine muscle cells of the rat before and after contraction

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Summary

Tunica muscularis of the rat's small intestine was studied electron microscopically before and after glycerol-extraction and at various times after ATP treatment. Before and after extraction only F-actin-filaments with a diameter of 50–80 Å could be found in smooth muscle cells. Dense bodies disappear during extraction. Glycerinated smooth muscle cells contract when ATP is added. At the same time thick filaments with a diameter of 150–200 Å appear, which probably represent myosin filaments, running longitudinally within the cells. During prolonged ATP treatment actin and myosin filaments first separate from each other by a sort of “sliding mechanism” because actin filaments are still bound to the cell membrane while myosin filaments move. Then actin filaments are drawn off from the cell membrane and actin and myosin filaments assemble in an intricate network of filaments in the central part of the cell. Around this network fine filaments with a diameter of 20–30 Å remain.

Zusammenfassung

Die Tunica muscularis des Dünndarms der Ratte wurde elektronenmikroskopisch vor und nach Glycerinextraktion und nach verschieden lang andauernder ATP-Behandlung untersucht. Vor und nach der Extraktion sind nur 50–80 Å breite F-Actin-Filamente in den glatten Muskelzellen nachzuweisen. Die extrahierten glatten Muskelzellen kontrahieren sich nach Zugabe von ATP. Gleichzeitig treten in der Längsrichtung der Zelle verlaufende 150–200 Å dicke Myosinfilamente auf. Während langanhaltender Inkubation mit ATP trennen sich Actin- und Myosinfilamente zunächst voneinander durch eine Art Gleitmechanismus, da die Actinfilamente noch an der Zellmembran verhaftet bleiben, die Myosinfilamente sich aber verschieben. Dann lösen sich die Actinfilamente von der Zellmembran und Actin- und Myosinfilamente bilden ein dichtes Netzwerk im Zentrum der Zelle. In der Umgebung dieses Netzwerkes verbleiben feine Filamente mit einem Durchmesser von 20–30 Å.

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  1. II. Anatomisches Institut der Freien Universität Berlin, Deutschland

    D. Graf Keyserlingk

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  1. D. Graf Keyserlingk
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Für die technische Mithilfe danke ich Frau Karla Struwe.

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Keyserlingk, D.G. Ultrastruktur glycerinextrahierter Dünndarmmuskelzellen der Ratte vor und nach Kontraktion. Z. Zellforsch. 111, 559–571 (1970). https://doi.org/10.1007/BF00330932

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  • DOI: https://doi.org/10.1007/BF00330932

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