Skip to main content
Log in

Immunofluorescence studies on the effect of cyclic adenosine 3′,5′-monophosphate in tissue culture preparations of the cervicovaginal anlage from neonatal mice

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

The authors have studied the production of antigenic material, specific for the cervicovaginal epithelium, in organ cultures of the cervicovaginal region from neonatal mice. In control cultures the antigenic material appeared at a time corresponding to the normal appearance under in vivo conditions. When cyclic adenosine 3′, 5′-monophosphate (dibutyryl derivate) was added to the medium, a strong increase of the fluorescent material was seen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Adiga, P. R., Murthy, P. V. N., McKenzie, J. M.: Stimulation by adenosine 3′, 5′-cyclic monophosphate of protein synthesis by adenohypophyseal polyribosomes. Biochemistry, 10, 711–715 (1971).

    Google Scholar 

  • Assan, R.: Le système de l'adényl-cyclase dans la distribution de l'énergie. Path. et Biol. 18, 489–497 (1970).

    Google Scholar 

  • Forsberg, J.-G., Kvinnsland, S.: The appearance and distribution of vaginal antigen during the differentiation of the cervicovaginal epithelium in normal and estradiol treated mice. In print. (1971).

  • —, Nord, L.: Immunfluorescence studies on the distribution of mouse uterine epithelial antigen in foetal, immature and adult mice. J. Embryol. exp. Morph. 21, 85–95 (1969).

    Google Scholar 

  • Hechter, O., Soifier, D.: Involvement of the adenyl cyclase 3′,5′-AMP system in steroid hormone action. In: Basic actions of sex steroids on targetorgans. Third Internat. Seminar on Reproductive Physiology and Sexual Endocrinology, Brussels, Basel-München-Paris-London-New York: S. Karger (1971).

    Google Scholar 

  • —, Yoshinaga, K., Halkerston, D. K., Birchall, K.: Estrogen-like anabolic effects of cyclic 3′,5′ adenosine monophosphate and other nucleotides in isolated rat uterus. Arch. Biochem. 122, 449–465 (1967).

    Google Scholar 

  • Kuo, Y. F., Greenyard, P.: Cyclic nucleotide-dependent protein kinase. Comparison of various histons as substrates for adenosine 3′,5′-monophosphate-dependent and guanosin 3′,5′-monophosphate dependent protein kinases. Biochem. Biophys. Acta 212, 434 (1970).

    Google Scholar 

  • Langan, T. A.: Histone phosphorylation: Stimulation by adenosine 3′,5′-monophosphate. Science 162, 579–580 (1968).

    Google Scholar 

  • Robison, G. A.: Cyclic AMP as a second messenger. J. Reprod. Fertil., Suppl. 10, 55 (1970).

    Google Scholar 

  • Rosenfeld, M. G., O'Malley, B. W.: Steroid hormones: Effects of adenyl cyclase activity and adenosine 3′,5′-monophosphate in target tissues. Science, 168–253 (1970).

  • Sharma, S. K., Talwar, G. P.: Action of cyclic adenosine 3′,5′-monophosphate in vitro on the uptake and incorporation of uridine into ribonucleic acid in ovariectomized rat uterus. J. biol. Chem. 245, 1513–1519 (1970).

    Google Scholar 

  • Stellwagen, R. H., Cole, R. D.: Chromosomal proteins. Ann. Rev. Biochem. 38, 951–990 (1969).

    Google Scholar 

  • Sutherland, E. W.: In: The Harvey lectures, p. 17–33. New York and London: Academic Press 1962.

    Google Scholar 

  • Szego, C. M., Davis, J. S.: Adenosine 3′,5′-monophosphate in rat uterus: Acute elevation by estrogen. Proc. nat. Acad. Sci. (Wash.) 58, 1711 (1967).

    Google Scholar 

  • Wicks, W.: Differential effects of glucocorticoids and adenosine 3′,5′-monophosphate on hepatic enzyme synthesis. J. biol. Chem. 246, 217–223 (1971).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This investigations was supported by grants from the Norwegian Research Council and the Norwegian Cancer Society (Landsforeningen mot Kreft).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fjellestad, D.H., Kvinnsland, S. Immunofluorescence studies on the effect of cyclic adenosine 3′,5′-monophosphate in tissue culture preparations of the cervicovaginal anlage from neonatal mice. Z. Zellforsch. 121, 69–73 (1971). https://doi.org/10.1007/BF00330917

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00330917

Key-Words

Navigation