Summary
DNA purified by a procedure based on phenol extraction contained many intact linkages, i.e. between genetic markers showing less than 20% cotransfer. The molecular weight of this DNA, as revealed by zone centrifugation, varied between 4.5×107 and 2.5×108. Linkages with cotransfer frequencies greater than 10% were found to consist of not more than 6.0×107 daltons of DNA, while one linkage showing only 2% cotransfer was provisionally estimated to be about 1.5×108 daltons. With the aim of extending the transformation map of B. subtilis, 16 mutations for nutritional requirements, not suspected—on the basis of the phenotypes involved—to be linked to any other markers, were examined for linkage with each other and to markers on the existing map. Three new pairs of distantly linked markers were found, but no linkage to any location on the known map.
The study of the linkage properties of markers which Oishi, Yoshikawa and Sueoka localized on their “replication map” suggests that some of these markers may have been misplaced.
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Kelly, M.S. Physical and mapping properties of distant linkages between genetic markers in transformation of Bacillus subtilis . Molec. Gen. Genetics 99, 333–349 (1967). https://doi.org/10.1007/BF00330909
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DOI: https://doi.org/10.1007/BF00330909