Summary
An assay for the detection of pyrimidine dimers has been adapted for use in permeabilized Neurospora crassa cells. Dimers are produced as a linear function of UV dose. Each Jm-2 of UV fluence yields 0.40 dimers per 108 daltons of DNA. Wild type Neurospora removes dimers during post-irradiation incubation. This excision repair process proceeds much more rapidly in nutritive medium (80% repair in 60 min) than in phosphate buffer (25% repair in 60 min). Cycloheximide added immediately after a damaging UV dose inhibits repair in growth medium but does not affect repair in phosphate buffer. A low dose of UV, 2 Jm-2, presented prior to the damaging dose, 22 Jm-2, increases excision repair activity in phosphate buffer. The data suggests that an inducible as well as a constitutive excision repair process is present in this eukaryotic organism.
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Communicated by Ch. Auerbach
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Baker, T.I. Inducible nucleotide excision repair in Neurospora . Mol Gen Genet 190, 295–299 (1983). https://doi.org/10.1007/BF00330654
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DOI: https://doi.org/10.1007/BF00330654