Summary
A number of cya and crp mutants of Escherichia coli HfrH were analyzed for several Tra functions of the F plasmid. The mutants were observed to be deficient in conjugal donor ability, absorption of phages MS2 and Qβ and surface exclusion. These defects were suppressed in cya mutants grown with cAMP supplementation. A cAMP concentration of 3×10-4 M produced maximal suppression of donor ability defect in a cya strain. cAMP did not suppress the Tra− phenotype of crp mutants. Latent periods of MS2 were shorter in cya and crp bacteria. Phage T7 development appeared similar in wild type, cya, and crp cells. It is concluded that tra genes of F plasmid are expressed only to a small extent in cya and crp mutants and that cAMP and its receptor protein are required for the normal expression of tra genes.
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Kumar, S., Srivastava, S. Cyclic AMP and its receptor protein are required for expression of transfer genes of conjugative plasmid F in Escherichia coli . Mol Gen Genet 190, 27–34 (1983). https://doi.org/10.1007/BF00330320
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DOI: https://doi.org/10.1007/BF00330320