Summary
The survival of UV-irradiated cholera phage e5 was found to increase when the host cells, Vibrio cholerae MAK757, were exposed to a low dose of UV irradiation before phage infection (Weigle reactivation), indicating the existence of a UV-inducible DNA repair pathway (SOS repair) in V. cholerae MAK757. The induction signal generated by UV irradiation was transient in nature and lasted about 20–30 min at 37°C. Maximal weigle reactivation of the phage was obtained when the host cells were irradiated with a UV dose of 16 J/m2. V. cholerae MAK757 was also found to possess efficient photoreactivation and host cell reactivation of UV-damaged DNA in phage e5.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams MH (1959) Bacteriophages. Interscience, New York
Campbell LA, Yasbin RE (1984) A DNA excision repair system for N. gonorrhoeae. Mol Gen Genet 193: 561–563
Defais M, Caillet-Fauquet P, Fox MS, Radman M (1976) Induction kinetics of mutagenic DNA repair activity in E. coli following ultraviolet irradition. Mol Gen Genet 148: 125–130
Devoret R (1981) Inducible error-prone repair and induction of prophage lambda in E. coli. Prog Nuclei Acid Res Mol Biol 26: 251–263
Devoret R (1978) Inducible error-prone repair: one of the cellular responses to DNA damage. Biochemie 60: 1135–1140
Devoret R, Blanco M, George J, Radman M (1975) Recovery of phage from ultraviolet damage. In: Hanawalt PC, Setlow RB (eds) Molecular mechanisms for repair of DNA. Plenum, New York, pp 155–171
Feiner RR, Hill RF (1963) Effect of basic dyes on host cell reactiation of ultraviolet-irradiated phage. Nature 200: 291–293
Ghosh A, Das J, Maniloff J (1977) Lack of repair of ultraviolet light damage in M. gallisepticum. J Mol Biol 116: 337–344
Ghosh A, Das J, Maniloff J (1978) Effect of acriflavine on ultraviolet inactivation of A. laidlawii. Biochim Biophys Acta 543: 570–575
Ghosh RK, Siddiqui KAI, Mukhopadhyay G, Ghosh A (1985) Evidence that a system similar to the recA system of E. coli exists in V. cholerae. Mol Gen Genet 200: 439–441
Goldberg I, Mekalanos JJ (1986) Cloning of the V. cholerae recA gene and construction of V. cholerae recA mutant. J Bacteriol 165: 715–722
Hatchard CG, Parker CA (1956) A new sensitive chemical actinometer: II. Potassium ferrioxalate as a standard chemical actinometer. Proc R Soc Lond, Series A 235: 518–536
Harm A (1974) Dark recovery of UV irradiated phage T1: II. Interpretation of the survival kinetics obtained under conditions of host-cell reactivation. Mutat Res 25: 3–14
Harm W (1980) Biological effects of ultraviolet radiation. Cambridge University Press, Cambridge, pp 118–121
Harm W, Rupert CS (1963) Infection of transformable cells of H. influenzae by bacteriophage and bacteriophage DNA. Z Vererbungslehre 94: 336–348
Keener SL, McNamee KP, McEntee K (1984) Cloning and characterization of recA genes from P. vulgaris, E. carotovora, S. Flexneri and E. coli B/r. J Bacteriol 160: 153–160
Little JW, Mount DW (1982) The SOS regulatory system of E. coli. Cell 29: 11–22
Notani NK, Setlow JE (1980) Inducible repair system in H. influenzae unaccompanied by mutation. J Bacteriol 143: 516–519
Palit BN, Das G, Das J (1983) Repair of ultraviolet light-induced DNA damage in cholera bacteriophages. J Gen Virol 64: 1749–1755
Radman M (1975) SOS repair hypothesis: Phenomenology of an inducible DNA repair which is accompanied by mutagenesis. In: Hanawalt PC, Setlow RB (eds) Molecular mechanisms for repair of DNA. Plenum, New York, pp 355–367
Sauerbier W (1964) Inhibition of host cell reactivation in phage T1 by caffeine. BIochem Biophys Res Commun 14: 340–346
Siddiqui KAI, Ghosh A (1983) Temperate phage induction and filament formation in V. cholerae by furazolidone. Biochem Biophys Res Commun 112: 1106–1111
Silva BS, Leitao AC (1984) UV induction of SOS responses in S. epidermis: Characteristics of the process. Photochem Photobiol 39: 781–785
Walker GC (1985) Inducible DNA repair systems. Annu Rev Biochem 54: 425–457
Weigle JJ (1953) Induction of mutation in bacterial virus. Proc Natl Acad Sci USA 39: 628–636
Yasbin RE (1985) DNA repair in Bacilus subtilis. In: Dubnau DA (ed) The molecular biology of the bacilli. Academic Press, New York, pp 33–52
Author information
Authors and Affiliations
Additional information
Communicated by R. Devoret
Rights and permissions
About this article
Cite this article
Basu, R., Ghosh, A. Inducible reactivation of UV-irradiated cholera phage e5 in Vibrio cholerae MAK757. Mol Gen Genet 209, 175–178 (1987). https://doi.org/10.1007/BF00329855
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00329855