Abstract
Using BrdU/Hoechst 33258/Giemsa methods for detecting replicating chromosome bands, a method is described by which the DNA synthesis phase may be sub-divided on the basis of distinctive patterns displayed by certain chromosomes. — Applied to asynchronous populations successively sampled through one cell cycle, cells in S can be “unscrambled” and replaced in their correct time sequence. — This helps to overcome the sampling-time variable inherent in such populations, and allows a clearer picture of the progression of events both qualitatively and quantitatively.
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Savage, J.R.K., Bhunya, S.P. Cytological sub-division of S-phase in the Syrian hamster (Mesocricetus auratus). Chromosoma 77, 169–180 (1980). https://doi.org/10.1007/BF00329542
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DOI: https://doi.org/10.1007/BF00329542