Summary
Four experimental protocols were completed to study the respiratory activity of isolated mitochondria from rats infected with Trypanosoma lewisi.
By Warburg respirometry, oxygen uptake was measured endogenously and with glucose, lactate, α-ketoglutarate, glutamate and succinate as substrates.
Respiratory activity of mitochondria isolated from rats infected with T. lewisi was greater than that from uninfected animals throughout the periods of observation (Day 1 to Day 40). In succinate, glutamate and α-ketoglutarate, respiratory activity of mitochondria from infected and uninfected rats was higher than in glucose.
A study of trypanosome populations during the 40 days of observation showed that the respiratory rate of mitochondria from infected animals could not be correlated with the number of parasites in the peripheral blood of the host. After the disappearance of trypanosomes from the host, the respiratory rate of mitochondria from infected rats was still significantly different from that of mitochondria from non-infected rats.
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References
Adler, S., Ashbell, R.: A note on the metabolism of tissues with Leishmania donovani. Ann. trop. Med. Parasit. 34, 207–210 (1940)
Blair, P. V., Sollars, F. A.: Contraction of hypotonically swollen mitochondria by oxidizable substrates. Biochem. biophys. Res. Commun. 27, (2), 202–208 (1967)
Brand, T. von: The gaseous exchange of trypanosome infected rats. Exp. Parasit. 1, 60–65 (1951)
Brandao, D., Gregori, N. X., Voss, D. O., Campello, A. P. L.: Strophocheilus oblongus: Heart mitochondria, respiration and oxidate phosphorylation. Exp. Parasit. 26, 203–208 (1969)
Chatterjee, A.: The liver in Trypanosoma evansi infection in white mice. Bull. Calcutta Sch. trop. Med. 8, 119–121 (1960)
Chatterjee, A., Mukherji, K. I., Sen Gupta, P. C.: Oxygen consumption of rat liver in Plasmodium berghei infection. Bull. Calcutta Sch. trop. Med. 7 (2), 61–63 (1957)
Chatterjee, A., Sen Gupta, P. C.: Studies on the liver of Leishmania infected hamsters. Bull. Calcutta Sch. trop. Med. 7 (3), 97–99 (1959)
Christie, G. S., Judah, J. D.: Mechanism of action of carbon tetrachloride on liver cells. Proc. roy. Soc. Biol. 142, 241 (1954)
Dawes, C. J.: Biological techniques in electron microscopy, 1st ed. New York: Barnes and Noble 1971
DeRobertis, E. D. P., Nowinski, W. W., Francisco, A. S.: Cell biology, 4th ed. Philadelphia: W. B. Saunders Co. 1968
Dickson, W. M., Dunlap, J. S., Johnson, V. L., Dunlap, D.: Oxygen uptake of liver slices from rabbits infected with Eimeria stiedae. Proc. Soc. exp. Biol. (N. Y.) 98, 179–180 (1958)
Fenyvessy, B. von: Über die Bedeutung des Stoffwechsels der Parasiten für das Wirtstier bei der Trypanosomen Infektion. Biochem. Z. 173, 289–297 (1926)
Henry, R. F., Sobel, C., Berkman, S.: Interferences with biuret methods for serum proteins. Use of Benedict's qualitative glucose reagent as biuret reagent. Ann. Chem. 29, 1491–1495 (1957)
Jones, E. S., Maegraith, B. G., Sculthorpe, H. H.: Pathological process in disease. III. The oxygen uptake of blood from albino rats infected with Plasmodium berghei. Ann. trop. Med. Parasit. 45, 244 (1951)
Kligler, I. J., Geiger, A., Komaroff, R.: Susceptibility and resistance to trypanosome infections. VII. Cause of injury and death in trypanosome infected rats. Ann. trop. Med. Parasit. 23, 325–335 (1929)
Kun, E., Volfin, P.: Metabolic differences between mitochondria isolated from various tissues. Biochem. biophys. Res. Commun. 23 (5), 696–701 (1966)
Lincicome, D. R., Bruce, J. I.: Oxygen uptake of liver and heart slices of Trypanosoma rhodesiense infected mice. Exp. Parasit. 17, 332–339 (1965)
Lincicome, D. R., Shepperson, J. R.: Experimental evidence for molecular exchanges between a dependent Trypanosome cell and its host. Exp. Parasit. 17, 148–167 (1965)
Lincicome, D. R., Watkins, R. C.: Method for preparing pure suspensions of Trypanosoma lewisi. Amer. Inst. Biol. Sci. Bull. 13, 53–54 (1963)
Maegraith, B. G., Riley, M. V., Deegan, T.: Changes in the metabolism of liver mitochondria of monkeys infected with Plasmodium knowlesi and their importance in the pathogenesis of malaria. Ann. trop. Med. Parasit. 56, 483–491 (1962)
Riley, M. V., Deegan, T.: The effect of Plasmodium berghei malaria on mouse liver mitochondria. Biochem. J. 76, 41–46 (1960)
Riley, M. V., Maegraith, B. G.: Changes in the metabolism of liver mitochondria of mice infected with rapid acute Plasmodium berghei. Ann. trop. Med. Parasit. 56, 473 (1962)
Roodyn, D. B.: Protein synthesis in mitochondria. Biochem. J. 85, 177 (1962)
Schaeff, C., Rabati, F.: Über die Einwirkung von Dinitrophenol auf den Energieumsatz und Sauerstoffverbrauch normaler und infizierter Mäuse. Biochem. Z. 298, 101–109 (1938)
Snedecor, G. W.: Statistical methods. Ames, Iowa: Iowa State College Press 1959
Umbreit, W. W., Burris, R. H., Stauffer, J. S.: Manometric techniques, 4th ed. Minneapolis: Burges 1964
Woods, M. W.: Polyvinylpyrrolidone (PVP) as adjunct in centrifugal separation and enzymic assay of subcellular components. Proc. Soc. exp. Biol. (N. Y.) 87, 71–73 (1954)
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Lee, C.M., Barnabas, E. Trypanosoma lewisi: Oxygen uptake of rat liver mitochondria. Z. Parasitenk. 43, 53–62 (1973). https://doi.org/10.1007/BF00329537
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DOI: https://doi.org/10.1007/BF00329537