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Asymmetric banding of Vicia faba chromosomes after BrdU incorporation

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Abstract

Growth of Vicia faba roots in BrdU for 17 h (about one cell cycle duration) followed by application of the FPG technique resulted in a characteristic pattern of asymmetric FPG bands, which occupy one or the other of the two sister chromatids of the metaphase chromosomes and are assumed to be indicative of clusters in chromosomal DNA with unequal Thd distribution between the complementary polynucleotide chains. Chromosomal position and visualization frequency of these bands have been established and compared with the banding patterns obtained after application of other techniques (fluorescence- and Giemsa technique, incorporation of azacytidine into chromosomal DNA, DNA late replication pattern). FPG bands and bands occurring after application of the techniques showed only limited positional coincidence. Some aspects of the FPG technique after BrdU incorporation have been used to make inferences with respect to the cell cycle parameters in the main root meristem of Vicia faba.

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Authors and Affiliations

  1. Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDR, 4325, Gatersleben, DDR

    I. Schubert & R. Rieger

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  1. I. Schubert
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  2. R. Rieger
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Schubert, I., Rieger, R. Asymmetric banding of Vicia faba chromosomes after BrdU incorporation. Chromosoma 70, 385–391 (1979). https://doi.org/10.1007/BF00328774

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  • DOI: https://doi.org/10.1007/BF00328774

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