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Cytogenic mapping and isolation of mutations of the β-Gal-1 locus of Drosophila melanogaster

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Summary

The structural gene β-Gal-1 encoding a β-galactosidase (EC 3.2.1.23) of Drosophila melanogaster has been mapped by two independent genetic approaches. In the first, gene dosage dependent variation in β-galactosidase activity levels in segmental aneuploids, generated from crosses of Y-autosome translocation stocks, was determined quantitatively. A dosage sensitive region on the left arm of chromosome 2 was identified and mapped to region 26A7-9. In the second approach, two null activity variants were isolated from wild populations. It was shown by deletion analysis that these nulls map to the same region as that determined by the segmental aneuploidy method. The results of an EMS mutagenesis screen showed that, besides the β-Gal-1 locus, there are four loci defined by recessive lethal mutations which map in the 26A7-9 region.

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References

  • Ashburner M (1973) Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster. I. Dependence upon ecdysone concentration. Dev Biol 35:47–61

    Google Scholar 

  • Ashburner M, Berendes HD (1978) Puffing of polytene chromosomes. In: Ashburner M, Wright TRF (eds) The Genetics and Biology of Drosophila, 2B. Academic Press, New York, pp 315–395

    Google Scholar 

  • Ashburner M, Richards G (1976) Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster. III. Consequences of ecdysone withdrawal. Dev Biol 54:241–255

    Google Scholar 

  • Becker HJ (1959) Die Puffs der Speicheldrüsenchromosomen von Drosophila melanogaster. I. Beobachtungen zum Verhalten des Puffmusters im Normalstamm und bei zwei Mutanten, giant and lethal giant larvae. Chromosoma (Berl) 10:654–678

    Google Scholar 

  • Bender W, Spierer P, Hogness DS (1983) Chromosome walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex of Drosophila melanogaster. J Mol Biol 168:17–33

    Google Scholar 

  • Berger E, Frank M, Abell MC (1980) Ecdysone induced changes in protein synthesis in embryonic Drosophila cells in culture. In: Kurstak E, Maramorosch K, Dubendorfer A (eds) Invertebrate systems in vitro. Elsevier North Holland, Amsterdam, pp 195–205

    Google Scholar 

  • Best-Belpomme M, Courgeon AM, Rambach A (1978) β-galactosidase is induced by hormone in Drosophila melanogaster cell cultures. Proc Natl Acad Sci USA 75:6102–6106

    Google Scholar 

  • Best-Belpomme M, Courgeon AM, Echalier G (1980) Development of a model for the study of ecdysteroid action: Drosophila melanogaster cells established in vitro. In: Hoffman JA (ed) Progress in ecdysone research. Elsevier/North Holland Biomedical Press, Amsterdam, pp 379–392

    Google Scholar 

  • Bingham PM, Judd BH (1981) Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell 25:693–704

    Google Scholar 

  • Bonner JJ, Pardue ML (1977) Ecdysone-stimulated RNA synthesis in salivary glands of Drosophila melanogaster: assay by in situ hybridization. Cell 12:219–225

    Google Scholar 

  • Bridges CB (1942) A new map of the salivary gland 2L-chromosome of Drosophila melanogaster. J Hered 33:403–408

    Google Scholar 

  • Doane WW (1973) Role of hormones in insect development. In: Counce SJ, Waddington CH (eds) Developmental systems: insects, vol 2. Academic Press, New York, pp 291–497

    Google Scholar 

  • Grell EH (1967) Electrophoretic variants of α-glycerophosphate dehydrogenase in Drosophila melanogaster. Science 158:1319–1320

    Google Scholar 

  • Ireland R, Berger E (1982) Sythesis of low molecular weight heat shock polypeptides stimulated by ecdysterone in a cultured Drosophila cell line. Proc Natl Acad Sci USA 79:855–859

    Google Scholar 

  • Ireland RC, Berger E, Sirotkin K, Yund MA, Osterbur D, Fristrom J (1982) Ecdysterone induces the transcription of four heat-shock genes in Drosophila S3 cells and imaginal discs. Dev Biol 93:498–507

    Google Scholar 

  • Kotarski MA, Pickert S, MacIntyre RJ (1983) A genetic and cytogenetic analysis of the α-glycerophosphate dehydrogenase region of Drosophila melanogaster. Genetics 105:371–386

    Google Scholar 

  • Langley CH, Voelker RA, Leigh-Brown AJ, Ohnishi S, Dickson B, Montgomery E (1981) Null allele frequencies at allozyme loci in natural populations of Drosophila melangogaster. Genetics 99:151–156

    Google Scholar 

  • Lewis E, Bacher F (1968) Method of feeding ethyl-methane-sulphonate (EMS) to Drosophila males. Drosophila Inform Serv 43:193

    Google Scholar 

  • Lindsley DL, Grell EH (1968) Genetic variations of Drosophila melanogaster. Carnegie Institute of Washington Publ No 627

  • Lindsley D, Sandler L, Baker BS, Carpenter ATC, Denell RE, Hall JC, Jacobs PA, Gabor-Miklos GL, Davis BK, Gethmann RC, Hardy RW, Hessler A, Miller SM, Nozawa H, Parry DM, Gould-Somero M (1972) Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics 71:157–184

    Google Scholar 

  • Lis JT, Simon JA, Sutton CA (1983) New heat shock puffs and β-galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid gene. Cell 35:403–410

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • MacIntyre RJ (1971) A method for measuring the activities of acid phosphatases separated by acrylamide gel electrophoresis. Biochem Genet 5:45–54

    Google Scholar 

  • Meyerwith EM, Hogness DS (1982) Molecular organization of a Drosophila puff site that responds to ecdysone. Cell 28:165–176

    Google Scholar 

  • Muskavitch MAT, Hogness DS (1980) Molecular analysis of a gene in a developmentally regulated puff of Drosophila melanogaste. Proc Natl Acad Sci USA 77:7362–7366

    Google Scholar 

  • O'Brien JS (1972) Ganglioside storage diseases. In: Harris H, Hirschhorn K (eds) Advances in human genetics, vol 3. Plenum Press, New York, pp 39–98

    Google Scholar 

  • O'Brien SJ, Gethmann R (1973) Segmental aneuploidy as a probe for structural genes in Drosophila — mitochondrial membrane enzymes. Genetics 75:155–167

    Google Scholar 

  • O'Brien SJ, MacIntyre RJ (1978) Genetics and biochemistry of enzymes and specific proteins of Drosophila. In: Ashburner M, Wright TRF (eds) The genetics and biology of Drosophila, vol 2A. Academic Press, New York, pp 396–551

    Google Scholar 

  • Paigen K (1979) Acid hydrolases as models of genetic control. Annu Rev Genet 13:417–466

    Google Scholar 

  • Pye Q, Knipple D, MacIntyre RJ (1980) Construction of segmental deficiency stocks from Y-autosome translocation stocks. Drosophila Inform Serv 55:170

    Google Scholar 

  • Rubin GM, Spradling AC (1982) Genetic transformation of Drosophila with transposable element vectors. Science 218:348–353

    Google Scholar 

  • Rpradling AC, Rubin GM (1982) Transposition of cloned P elements into Drosophila germ line chromosomes. Science 218:341–347

    Google Scholar 

  • Velissariou V, Ashburner M (1980) The secretory proteins of the larval salivary gland of Drosophila melanogaster. Chromosoma 77:13–27

    Google Scholar 

  • Voelker RA, Langley CH, Leigh-Brown AJ, Ohnishi S, Dickson B, Montgomery E, Smith SC (1980) Enzyme null alleles in natural populations of Drosophila melanogaster: frequencies in a North Carolina population. Proc Natl Acad Sci USA 75:1091–1095

    Google Scholar 

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Author notes

  1. Douglas C. Knipple

    Present address: Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35/II, D-7400, Tübingen, Federal Republic of Germany

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  1. Section of Genetics and Development, Cornell University, 14853, Ithaca, NY, USA

    Douglas C. Knipple & Ross J. MacIntyre

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  1. Douglas C. Knipple
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  2. Ross J. MacIntyre
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Communicated by M.M. Green

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Knipple, D.C., MacIntyre, R.J. Cytogenic mapping and isolation of mutations of the β-Gal-1 locus of Drosophila melanogaster . Molec Gen Genet 198, 75–83 (1984). https://doi.org/10.1007/BF00328704

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  • DOI: https://doi.org/10.1007/BF00328704

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