Summary
An analysis of nerve growth factor (NGF) receptor expression and density in human sural nerve biopsies was performed by immunocytochemistry with a murine monoclonal antibody against the human NGF receptor. Quantitative assessment of immunostaining density was made by histospectrophotometry on frozen sections. Although there was enhanced expression of NGF receptor within endoneurium in all patients with clinical neuropathies, expression was highest in nerves with axonal disease, consistent with the proposal that disruption of axon-Schwann cell interactions triggers the reexpression of the NGF receptor. These results with human nerves, together with previous studies with animal models, suggest that NGF and NGF receptor play important roles in the general response to neuronal injury.
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Bresolin N, Moggio M, Bet L, Gallanti A, Prelle A, Nobile-Orazio E, Adobbati L, Ferrante C, Pellegrini G, Scarlato G (1987) Progressive cytochrome c oxidase deficiency in a case of Kearns-Sayre syndrome: morphological, immunological, and biochemical studies in muscle biopsies and autopsy tissues. Ann Neurol 21:564–572
Distefano PS, Johnson EM Jr (1988) Nerve growth factor receptors on cultured Schwann cells. J Neurosci 8:231–241
Draper N, Smith H (1981) Applied regression analysis, 2nd edn. Wiley, New York
Dyck PJ, Karnes J, Lais A, Lofgren EP, Stevens JC (1984) Pathologic alteration of the peripheral nervous system of humans. In: Dyck PJ, Thomas PK, Lambert EH, Bunge R (eds) Peripheral neuropathy, 2nd edn. Saunders, Philadelphia
Godfrey EW, Shooter EM (1986) Nerve growth factor receptors on chick embryo sympathetic ganglion cells: binding characteristics and development. J Neurosci 6:2543–2550
Heumann R, Korsching S, Bandtlow C, Thoenen H (1987) Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection. J Cell Biol 104:1623–1631
Hosang M, Shooter EM (1985) Molecular characteristics of nerve growth factor receptors on PC12 cells. J Biol Chem 260:655–662
Johnson EM, Rich KM, Yip HK (1986) The role of NGF in sensory neurons in vivo. Trends Neurosci 9:33–37
Levi-Montalcini R, Angeletti PU (1963) Essential role of the nerve growth factor in survival and maintenance of dissociated sensory and sympathetic embryonic nerve cell in vitro. Dev Biol 7:653–659
Mokuno K, Yasuda T, Sobue G, Kreider B, Ross A, Pleasure D (1987) Rat Schwann cells express NGF receptors which are down-regulated by cyclic AMP. Neurology 37 [Suppl 1]:235
Ohi T, Kyle RA, Dyck PJ (1985) Axonal attenuation and secondary segmental demyelination in myeloma neuropathies. Ann Neurol 17:255–261
Pleasure D, Kreider B, Sobue G, Ross AH, Koprowski H, Sonnenfeld KH, Rubenstein AE (1986) Schwann-like cells cultured from human dermal neurofibromas: immunohistological identification and response to Schwann cell mitogens. Ann NY Acad Sci 486:227–240
Raivich G, Zimmermann A, Sutter A (1985) The spatial and temporal pattern of NGF receptor expression in the developing chick embryo. EMBO 4:637–644
Rohrer H (1985) Nonneuronal cells from chick sympathetic and dorsal root sensory ganglia express catecholamine uptake and receptors for nerve growth factor during development. Dev Biol 111:95–107
Ross AH, Grob P, Bothwell M, Elder de, Ernst CS, Marano N, Ghrist BFD, Slemp CC, Herlyn M, Atkinson B, Koprowski H (1984) Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. Proc Nat Acad Sci USA 81:6681–6685
Ross AH, Pleasure D, Sonnenfeld K, Atkinson B, Kreider B, Jackson DM, Taff I, Scarpini E, Lisak RP, Koprowski H (1986) Expression of melanoma-associated antigens by normal and neurofibroma Schwann cells. Cancer Res 46:5887–5892
Scarpini E, Ross AH, Rosen JL, Brown MJ, Rostami A, Koprowski H, Lisak RP (1988) Expression of nerve growth factor receptor during human peripheral nerve development. Dev Biol 125:301–310
Seilheimer B, Schachner M (1987) Regulation of neural cell adhesion molecule expression on cultured mouse Schwann cells by nerve growth factor. EMBO 6:1611–1616
Sobue G, Yasuda T, Mitsuma T, Ross AH, Pleasure D (1988) Expression of nerve growth factor receptor in human peripheral neuropathies. Ann Neurol 24:64–72
Taniuchi M, Clark HB, Johnson EM (1986) Induction of nerve growth factor receptor in Schwann cells after axotomy. Proc Nat Acad Sci USA 83:4094–4098
Taniuchi M, Clark HB, Schweitzer JB, Johnson EM (1988) Expression of nerve growth factor receptors by Schwann cells of axotomized peripheral nerves: ultrastructural location, suppression by axonal contact, and binding properties. J Neurosci 8:664–681
Yan Q, Johnson EM Jr (1987) A quantitative study of the developmental expression of nerve growth factor (NGF) receptor in rats. Dev Biol 121:139–148
Yankner BA, Shooter EM (1982) The biology and mechanism of action of nerve growth factor. Ann Rev Biochem 51:845–868
Yasuda T, Sobue G, Mokuno K, Kreider B, Pleasure D (1987) Cultured rat Schwann cells express low-affinity receptors for nerve growth factor. Brain Res 436:113–120
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Scarpini, E., Beretta, S., Ross, A.H. et al. Rapid quantitative immunohistochemical assessment of human peripheral neuropathies using a monoclonal antibody against nerve growth factor receptor. J Neurol 236, 439–444 (1989). https://doi.org/10.1007/BF00328502
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DOI: https://doi.org/10.1007/BF00328502