Abstract
Polytene chromosome sections 63E1-6 of 3L in Drosophila melanogaster were studied by 3H-uridine and 3H-thymidine autoradiography in late third instar larvae and prepupae. In late third instar larvae 63E does not incorporate 3H-uridine. In prepupae, however, a large puff is formed in 63E which is most active in RNA synthesis. — 3H-thymidine labeling patterns and frequencies of regions 61A-64C were analysed and the non-puffed and puffed 63E sections were compared with reference sections. Both in late third instar larvae and in prepupae 63E shows late replication behavior. It is concluded that the decondensation of chromosome bands does not necessarily entail earlier and/or faster DNA replication.
Similar content being viewed by others
References
Ananiev, E.V., Gvozdev, V.A.: Differences in DNA replication patterns in the X-chromosome of males, females and intersexes of Drosophila melanogaster. Chromosoma (Berl.) 49, 233–241 (1975)
Arcos-Terán, L.: DNS-Replikation und die Natur der spät replizierenden Orte im X-Chromosom von Drosophila melanogaster. Chromosoma (Berl.) 37, 233–296 (1972)
Ashburner, M.: Patterns of puffing activity in the salivary gland chromosomes of Drosophila. I. Autosomal puffing patterns in a laboratory stock of Drosophila melanogaster. Chromosoma (Berl.) 21, 398–428 (1967)
Becker, H.J.: Die Puffs der Speicheldrüsenchromosomen von Drosophila melanogaster. I. Mitteilung. Beobachtungen zum Verhalten des Puffmusters im Normalstamm und bei zwei Mutanten, giant und lethal-giant-Larvae. Chromosoma (Berl.) 10, 654–678 (1959)
Berendes, H.D.: Differential replication of male and female X-chromosomes in Drosophila. Chromosoma (Berl.) 20, 32–43 (1966)
Chatterjee, S.N., Mukherjee, A.S.: Chromosomal basis of dosage compensation in Drosophila. VIII. DNA replication patterns of the puffs in the male and female larval polytene chromosomes. Cell Differentiation 2, 1–19 (1973)
Chatterjee, S.N., Mukherjee, A.S.: DNA replication in polytene chromosomes of Drosophila pseudoobscura: New facts and their implications, I. J. exp. Biol. 13, 452–459 (1975)
Gabrusewycz-Garcia, N.: Cytological and autoradiographic studies in Sciara coprophila salivary gland chromosomes. Chromosoma (Berl.) 15, 312–344 (1964)
Hägele, K.: DNS-Replikationsmuster der Speicheldrüsenchromosomen von Chironomiden. Chromosoma (Berl.) 31, 91–138 (1970)
Hagele, K.: Komplementäre DNA-Replikationsmuster bei Drosophila melanogaster. Chromosoma (Berl.) 41, 231–236 (1973)
Hagele, K., Kalisch, W.-E.: Initial phases of DNA synthesis in Drosophila melanogaster. I. Differential participation in replication of the X chromosomes in males and females. Chromosoma (Berl.) 47, 403–413 (1974)
Kalisch, W.-E., Hägele, K.: Different DNA replication behavior of a tandem duplication in Drosophila melanogaster. Chromosoma (Berl.) 44, 265–283 (1973)
Kalisch, W.-E., Hägele, K.: Correspondence of banding patterns to 3H-thymidine labeling patterns in polytene chromosomes. Chromosoma (Berl.) 57, 19–23 (1976)
Kalisch, W.-E., Hägele, K.: Determination of 3H-thymidine background labeling over polytene chromosomes in Drosophila melanogaster. Dros. Inf. Serv. 52, 127–128 (1977)
Keyl, H.-G., Pelling, C.: Differentielle DNS-Replikation in den Speicheldrüsenchromosomen von Chironomus thummi. Chromosoma (Berl.) 14, 347–359 (1963)
Lakhotia, S.C., Mukherjee, S.A.: Chromosomal basis of dosage compensation in Drosophila. III. Early completion of replication by the polytene X-chromosome in male: Further evidence and its implications. J. Cell Biol. 47, 18–33 (1970)
Lindsley, D.L., Grell, E.H.: Genetic variations of Drosophila melanogaster. Carnegie Inst. Wash. Publ. 627 (1968)
Mukherjee, A.S., Chatterjee, S.N.: Hyperactivity and faster replication property of the two arms of the male X of Drosophila pseudoobscura. J. Microscopy 106, 199–208 (1976)
Plaut, W., Nash, D., Fanning, T.: Ordered replication of DNA in polytene chromosomes of Drosophila melanogaster. J. molec. Biol. 16, 85–93 (1966)
Rodman, T.C.: Relationship of developmental stage to initiation of replication in polytene nuclei. Chromosoma (Berl.) 23, 271–287 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hägele, K., Kalisch, W.E. DNA replication of a polytene chromosome section in Drosophila prior to and during puffing. Chromosoma 79, 75–83 (1980). https://doi.org/10.1007/BF00328474
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00328474