Abstract
Individual bivalents or chromosomes have been identified in Drosophila melanogaster spermatocytes at metaphase I, anaphase I, metaphase II and anaphase II in electron micrographs of serial sections. Identification was based on a combination of chromosome volume analysis, bivalent topology, and kinetochore position. — Kinetochore microtubule numbers have been obtained for the identified chromosomes at all four meiotic stages. Average numbers in D. melanogaster are relatively low compared to reported numbers of other higher eukaryotes. There are no differences in kinetochore microtubule numbers within a stage despite a large (approximately tenfold) difference in chromosome volume between the largest and the smallest chromosome. A comparison between the two meiotic metaphases (metaphase I and metaphase II) reveals that metaphase I kinetochores possess twice as many microtubules as metaphase II kinetochores. — Other microtubules in addition to those that end on or penetrate the kinetochore are found in the vicinity of the kinetochore. These microtubules penetrate the chromosome rather than the kinetochore proper and are more numerous at metaphase I than at the other division stages.
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Baker, B.S., Carpenter, A.T.C.: Genetic analyses of sex chromosomal meiotic mutants in Drosophila melanogaster. Genetics 71, 255–286 (1972)
Baker, B.S., Hall, J.C.: Meiotic mutants: genetic control of meiotic recombination and chromosome segregation. In: The genetics and biology of Drosophila, (M. Ashburner and E. Novitski, eds), vol. 1a, pp. 351–434. New York: Academic Press 1976
Baker, B.S., Carpenter, A.T.C., Esposito, M.S., Esposito, R.E., Sandler, L.: The genetic control of meiosis. Ann. Rev. Genet. 10, 53–134 (1976)
Bridges, C.B.: Nodisjunction as proof of the chromosomal theory of inheritance. Genetics 1, 1–52, 107–163 (1916)
Cooper, K.W.: Meiotic conjunctive elements not involving chiasmata. Proc. nat. Acad, Sci. (Wash.) 52, 1248–1255 (1964)
Cooper, K.W.: Normal spermatogenesis in Drosophila. In: Biology of Drosophila (M. Demerec, ed.), pp. 1–61. New York, London: Hafner 1965
Forer, A., Brinkley, B.R.: Microtubule distribution in the anaphase spindle of primary spermatocytes of a crane fly (Nephrotoma suturalis). Canad. J. Genet. Cytol. 18, 503–519 (1977)
Fuge, H.: Ultrastructure and function of the spindle apparatus microtubules and chromosomes during nuclear division. Protoplasma (Wien) 82, 289–320 (1974)
Fuge, H.: Ultrastructure of mitotic cells. In: Mitosis, facts and questions. (M. Little, N. Petzelt, H. Ponstingl, D. Schroeter and H.P. Zimmerman, eds.), pp. 51–77, Berlin, Heidelberg, New York: Springer 1977
Fuge, H.: Microtubule disorientation in anaphase half-spindles during autosome segregation in crane fly spermatocytes. Chromosoma (Berl.) 76, 309–328 (1980)
Gassner, G.: Synaptinemal complexes in achiasmatic spermatogenesis of Bolbe nigra Giglio-Tos. (Mantoidea). Chromosome (Berl.) 26, 22–34 (1969)
Grell, R.F.: Distributive pairing. In: The genetics and biology of Drosophila (M. Ashburner, and E. Novitski., eds.). vol 1a, pp. 435–486. New York: Academic Press 1976
Hinton, T.: A comparative study of certain heterochromatic regions in the mitotic and salivary gland chromosomes of Drosophila melanogaster. Genetics 27, 119–127 (1942)
Jensen, C., Bajer, A.: Spindle dynamics and arrangements of microtubules. Chromosoma (Berl.) 44, 73–89 (1973)
Kaufmann, B.P.: Somatic mitoses of Drosophila melanogaster. J. Morph. 56, 125–155 (1934)
Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137a-138a (1965)
LaFountain, J.R., Jr.: Birefringence and fine structure of spindles in spermatocytes of Nephrotoma suturalis at metaphase of first meiotic division. J. Ultrastruct. Res. 46, 268–278 (1974)
Lindsley, D.L., Grell, E.H.: In: Genetic variations of Drosophila melanogaster. Carnegie Inst. Wash. publication no. 627 1967
Luft, J.H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409 (1961)
Maguire, M.P.: The need for a chiasma binder. J. theor. Biol. 48, 485–487 (1976)
Maguire, M.P.: An indirect test for a role of the synaptonemal complex in chiasma maintenance. Exp. Cell Res. 112, 297–308 (1978)
McGill, M., Brinkley, B.R.: Human chromosomes and centrioles as nucleating sites for in vitro assembly of microtubules from bovine brain tubulin. J. Cell Biol. 67, 189–199 (1975)
Meyer, G.F.: Die Funktionsstrukturen des Y-Chromosoms in den Spermatocytenkernen von Drosophila hydei, D. neohydei, D. repleta und einigen anderen Drosophila-Arten. Chromosoma (Berl.) 14, 207–255 (1963)
Moens, P.B.: Serial sectioning in electron microscopy. Proc. Canad. Fed. Biol. Soc. 13, 160 (1970)
Moens, P.B.: Kinetochore microtubule numbers of different sized chromosomes. J. Cell Biol. 83, 556–561 (1979)
Nicklas, R.B., Brinkley, B.R., Pepper, D.A., Kubai, D.K., Richards, G.K.: Electron microscopy of spermatocytes previously studied in life. Methods and some observations on micromanipulated chromosomes. J. Cell Sci. 35, 87–104 (1979)
Peachy, L.D.: Thin sections: I. A study of section thickness and physical distortion produced during microtomy. J. biophys. biochem. Cytol. 4, 233–242 (1958)
Pepper, D.A., Brinkley, B.R.: Microtubule initiation at kinetochores and centrosomes in lysed mitotic cells: inhibition of site specific nucleation by tubulin antibody. J. Cell Biol. 82, 585–591 (1979)
Rasmussen, S.W.: Ultrastructural studies of spermatogenesis in D. melanogaster Meigen. Z. Zellforsch. 140, 125–144 (1973)
Reynold, E.S.: The uses of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)
Riva, A.: A simple and rapid staining method for enhancing the contrast of tissues previously treated with uranyl acetate. J. Microscopie 19, 105–108 (1974)
Rudkin, G.T.: Photometric measurements of individual metaphase chromosomes. In Vitro 1, 12–20 (1965)
Sandler, L., Lindsley, D.L., Nicoletti, B., Trippa, G.: Mutants affecting meiosis in natural populations of Drosophila melanogaster. Genetics 60, 525–558 (1968)
Stanley, H.P., Bowman, S.T., Romrell, L.J., Reed, S.C., Wilkinson, R.F.: Fine structure of normal spermatid differentiation in Drosophila melanogaster. J. Ultrastruct. Res. 41, 433–466 (1972)
Tippit, D.H., Pickett-Heaps, J.D., Leslie, R.: Cell division in two large pennate diatoms Hantzschia and Nitzschia. III. A new proposal for kinetochore function during prometaphase. J. Cell Biol. 86, 402–416 (1980)
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Lin, H.P.P., Ault, J.G. & Church, K. Meiosis in Drosophila melanogaster . Chromosoma 83, 507–521 (1981). https://doi.org/10.1007/BF00328276
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DOI: https://doi.org/10.1007/BF00328276