Summary
Discrepancies between our mapping data concerning the cdd and gat marker of Escherichia coli and data obtained by Josephsen et al. (1983) as well as with the current linkage map of Escherichia coli (Bachmann 1983) led us to reinvestigate the mapping of markers in the gyrA-his region by P1 transduction. In addition, we isolated Hfr strains by integrating the temperature-sensitive F'ts114 lac + episome via lac homology of cir'lacZ and mgl'lacZ fusions into the chromosome. From the results of the P1 transductions and using these Hfr strains as donors in crosses it became clear that the transcription of cir and mgl is the same and counterclockwise on the chromosome, with cdd and gat as the counterclockwise markers to mgl and cir. We conclude that our previously published sequence of markers was incorrect and is in fact inverted. The present data indicate the following sequence of markers in clockwise order: his gat cdd mgl cir fpk gyrA.
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References
Argast M, Ludtke D, Silhavy TJ, Boos W (1978) A second transport system for sn-glycerol-3-phosphate in Escherichia coli. J Bacteriol 136:1070–1083
Bachmann B (1983) Linkage map of Escherichia coli K12, edition 7. Microbiol Rev 47:180–230
Boos W, Steinacher I, Engelhardt-Altendorf D (1981) Mapping of mglB, the structural gene of the galactose-binding protein of Escherichia coli. Mol Gen Genet 184:508–518
Boos W, Bantlow C, Benner D, Roller E (1983) cir, a gene conferring resistance to colicin I maps between mgl and fpk on the Escherichia coli chromosome. Mol Gen Genet 191:401–406
Bowles LK, Minguel AG, Konisky J (1983) Purification of the colicin I receptor. J Biol Chem 258:1215–1220
Bremer E, Silhavy TJ, Weisemann JM, Weinstock GM (1984) λplacMu: a transposable derivative of phage λ for creating lacZ protein fusions in a single step. J Bacteriol 158:1084–1093
Casadaban M (1976) Transposition and fusion of the lac genes to selected promotors in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol 104:541–555
Chumley FC, Menzel R, Roth JR (1979) Hfr formation directed by Tn10. Genetics 91:639–635
Clark AF, Hogg RW (1981) High affinity arabinose transport mutants of Escherichia coli: Isolation and gene location. J Bacteriol 147:920–924
Davis RW, Botstein D, Roth JR (1980) Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Ferenci T, Kornberg HL (1974) The role of the phosphotransferase mediated syntheses of fructose-1-phosphate and fructose-6-phosphate in the growth of Escherichia coli on fructose. Proc Soc London 187:105–119
Fuchs JA, Karlström HO (1976) Mapping of nrdA and nrdB in Escherichia coli K12. J Bacteriol 128:810–814
Hammer-Jespersen K, Munch-Petersen A (1973) Mutants of Escherichia coli unable to metabolize cytidine: isolation and characterization. Mol Gen Genet 126:177–186
Jones-Mortimer MC, Kornberg HL (1974) Genetic analysis of fructose utilization by Escherichia coli. Proc R Soc London B 187:121–131
Josephsen J, Hammer-Jespersen K, Hansen TD (1983) Mapping of the gene for cytidine deaminase (cdd) in Escherichia coli K12. J Bacteriol 154:72–75
Kleckner N, Chan R, Tye K, Botstein D (1975) Mutagenesis by insertion of a drug resistant element carrying an inverted repetition. J Mol Biol 97:561–575
Kleckner N, Roth JR, Botstein D (1977) Genetic engineering in vivo using translocatable drug resistance elements. J Mol Biol 116:125–159
Lengeler J (1975) Mutations affecting transport of the hexitols, D-mannitol, D-glucitol, and galactitol in Escherichia coli K12: Isolation and mapping. J Bacteriol 124:26–38
Link CD, Reiner AM (1983) Genotypic exclusion: a novel relationship between the ribitol-arabitol and galactitol genes of E. coli. Mol Gen Genet 189:337–339
Ludtke D, Larson TJ, Beck C, Boos W (1982) Only one gene is required for the glpT dependent transport of sn-glycerol-3-phosphate in Escherichia coli. Mol Gen Genet 186:540–547
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Neuhard J, Thomassen E (1976) Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K12 due to deletion of the dcd gene. J Bacteriol 126:999–1001
Parkinson JS, Houts SE (1982) Isolation and behavior of Escherichia coli deletion mutants lacking chemotaxis functions. J Bacteriol 151:106–113
Schnier J, Isono K (1979) The gene for ribosomal protein L25 (rplY) maps at 47.3 min near nalA in Escherichia coli K-12. Mol Gen Genet 176:313–318
Studier FW (1975) Genetic mapping of a mutation that causes ribonuclease III deficiency in Escherichia coli. J Bacteriol 124:307–316
Sunshine MG, Kelly B (1971) Extent of host deletions associated with bacteriophage P2-mediated eduction. J Bacteriol 108:695–704
Tabor H, Hafner EW, Tabor CW (1980) Construction of an Escherichia coli strain unable to synthesize putrescine, spermidine or cadaverine: characterization of two genes controlling lysine decarboxylase. J Bacteriol 144:952–956
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Middendorf, A., Schweizer, H., Vreemann, J. et al. Mapping of markers in the gyrA-his region of Escherichia coli . Mol Gen Genet 197, 175–181 (1984). https://doi.org/10.1007/BF00327939
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DOI: https://doi.org/10.1007/BF00327939