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Genes for gentamicin-(3)-N-acetyltransferases III and IV:

I. Nucleotide sequence of the AAC(3)-IV gene and possible involvement of an IS140 element in its expression

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Summary

Genes for gentamicin-3-acetyltransferases [ACC(3)] of types III and IV have been cloned from various R-plasmids. In two R-plasmids, pWP14a (AAC(3)-III) and pWP7b [AAC(3)-IV], resistance genes have been found directly adjacent to a single copy of an IS element, IS140. Nucleotide sequence determination of the AAC(3)-IV gene from plasmid pWP7b and of part of IS140 from three different sources suggested that the-35 region of the AAC(3)-IV promoter was part of the IS element. A similarly built-up promoter was found in pWP14a.

It was found also, that a hygromycin B phosphotransferase was expressed from a locus neighbouring the AAC(3)-IV gene in pWP7b which was under the control of the same promoter.

In two other R-plasmids, pWP113a and pWP116a, the AAC(3)-III gene was found in different genetic environments, namely close to Tn3-like structures.

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Authors and Affiliations

  1. Lehrstuhl für Mikrobiologie der Universität, Maria-Ward-Straße 1a, D-8000, München 19

    Barbara Bräu, Ursula Pilz & Wolfgang Piepersberg

Authors
  1. Barbara Bräu
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  2. Ursula Pilz
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  3. Wolfgang Piepersberg
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Communicated by K. Isono

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Bräu, B., Pilz, U. & Piepersberg, W. Genes for gentamicin-(3)-N-acetyltransferases III and IV:. Molec. Gen. Genet. 193, 179–187 (1984). https://doi.org/10.1007/BF00327434

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  • DOI: https://doi.org/10.1007/BF00327434

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