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Genetic control of nitrate reduction in the cyanobacterium nostoc muscorum

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Summary

There were three kinds of chlorate resistant (Clr-R) mutants: Clr-R1 type did not show nitrate reductase activity, Clr-R2 type was deficient in nitrate uptake activity and Clr-R3 type was defective in both. It is suggested that the genetic determinant of the uptake system is distinct from that of the reductase system.

The NH +4 -repressible uptake system showed a requirement of nitrate as an activator of its activity. In comparison, NH +4 -repressible reductase enzyme showed requirement of nitrate neither as an inducer of its synthesis nor as an activator of its activity. Thus, the available mutational and physiological evidence suggest no involvement of the nitrate uptake system in control of nitrate reductase activity and vice versa.

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Abbreviations

Tricine:

(N-tris[Hydroxymethyl] methyl glycine)

Tris:

Tris (Hydroxymethyl) amino methane

OD:

Optical Density

Chl.:

Chlorophyll

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Authors and Affiliations

  1. School of Life Sciences, University of Hyderabad, 500 134, Hyderabad, India

    S. N. Bagchi & H. N. Singh

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  1. S. N. Bagchi
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  2. H. N. Singh
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Communicated by H. Böhme

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Bagchi, S.N., Singh, H.N. Genetic control of nitrate reduction in the cyanobacterium nostoc muscorum . Molec. Gen. Genet. 193, 82–84 (1984). https://doi.org/10.1007/BF00327418

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  • DOI: https://doi.org/10.1007/BF00327418

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