Abstract
Galactose feeding of Chironomus thummi larvae induces the regression of Balbiani ring c (BRc) and the full expansion of BRb, both localized in the IV salivary gland chromosome. This effect coincides with that described on BR2 and BR1 of Ch. pallidivittatus and Ch. tentans. The puffing changes of BRb and BRc throughout development have been studied and also show identical variations as in BR1 and BR2 of Ch. pallidivittatus and Ch. tentans. The similar behaviour of BRb and BR1, and of BRc and BR2 respectively after galactose treatment and throughout development strongly suggests that these BRs play the same physiological role in the three Chironomus species, with BRb = BR1 and BRc=BR2.
Similar content being viewed by others
References
Bauer, H.: Der Aufbau der Chromosomen aus den Speicheldrüsen von Chironomus thummi. Kiefer (Untersuchungen an den Riesenchromosomen der Dipteren. I.). Z. Zellforsch. 23, 280–313 (1935)
Beermann, W.: Effect of α-amanitine of puffing and intranuclear RNA synthesis in Chironomus salivary glands. Chromosoma (Berl.) 34, 152–167 (1971)
Beermann, W.: Directed changes in the pattern of Balbiani ring puffing in Chironomus: Effects of a sugar treatment. Chromosoma (Berl.) 41, 297–326 (1973)
Colman, O.D., Stockert, J.C.: Puffing patterns during the fourth larval instar Chironomus pallidivittatus salivary glands. Chromosoma (Berl.) 53, 381–392 (1975)
Hägele, K.: Chironomus. In: Handbook of genetics (R.C. King, ed.), Vol. 3, pp. 269–278. Northwestern University Press 1975
Keyl, H.G.: Untersuchungen am Karyotypus von Chironomus thummi. I. Mitteilung. Karte der Speicheldrüsen-Chromosomen von Chironomus thummi thummi und die cytologische Differenzierung der Subspezies Ch. th. thummi und Ch. th. piger. Chromosoma (Berl.) 8, 739–756 (1957)
Lambert, B., Beermann, W.: Homology of Balbiani Ring DNA in Two Closely Related Chironomus Species. Chromosoma (Berl.) 51, 41–47 (1975)
Laufer, H., Holt, T.K.H.: Juvenile hormone effects on chromosomal puffing and development in Chironomus thummi. J. exp. Zool. 173, 341–352 (1970)
Lezzi, M., Gilbert, L.I.: Control of gene activities in the polytene Chromosomes of Chironomus tentans by ecdysone and juvenile hormone. Proc. nat. Acad. Sci. (Wash.) 64, 498–503 (1969)
Pankow, W., Lezzi, M., Holderegger-Mähling, I.: Correlated changes of Balbiani ring expansion and secretory protein synthesis in larval salivary glands of Chironomus tentans. Chromosoma (Berl.) 58, 137–153 (1976)
Rydlander, L., Edström, J.E.: Expression of Balbiani ring genes. Proc. 10th. FEBS-Meeting, pp. 149–156 (1975)
Serfling, E.: The transcripts of Balbiani rings from Chironomus thummi. Giant RNA molecules with messenger characteristics. Chromosoma (Berl.) 57, 271–283 (1976)
Serfling, E., Maximovsky, L.F., Wobus, U.: Synthesis and processing of ribosomal ribonucleic acid in salivary glands cells of Chironomus thummi. Europ. J. Biochem. 45, 277–289 (1974)
Serfling, E., Panitz, R., Wobus, U.: Die experimentelle Beeinflussung des Puffmusters von Riesenchromosomen. I. Puffinduktion durch Oxytetracyclin bei Chironomus thummi. Chromosoma (Berl.) 28, 107–119 (1969)
Stevens, B.J., Swift, H.: RNA transport from nucleus to cytoplasm in Chironomus salivary glands. J. Cell Biol. 31, 55–77 (1966)
Wobus, U., Panitz, R., Serfling, E.: Tissue specific gene activities and proteins in the Chironomus salivary gland. Molec. gen. Genet. 107, 215–223 (1970)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Santa-Cruz, M.C., Villanueva, A. & Díez, J.L. Effect of galactose treatment in the puffing pattern of Chironomus thummi Balbiani rings. Chromosoma 69, 93–100 (1978). https://doi.org/10.1007/BF00327383
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00327383