Abstract
Different transgenic plants of Brassica napus, Brassica nigra, Datura innoxia and Vicia narbonensis expressing the hph gene under the control of the 35s promoter were co-cultivated with mycelial material of Aspergillus niger in microcosms under sterile conditions. A significantly higher number of hygromycin B-resistant colonies of re-isolated fungi was obtained if compared with co-cultures with non-transgenic plants. The hph gene and other foreign sequences could be detected in some of the resistant strains only for a short time after selection, indicating a rapid loss of foreign DNA. A more stable transgenic strain was obtained after co-culture with transgenic plants of D. innoxia including a high number of hph copies in their genome. DNA with detected pUC sequences was prepared to transform E. coli DH5α. One of the recovered plasmids is shown to include pieces of the plant-transforming vector and a foreign sequence. The 35s-regulated expression of genes is studied in A. niger.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aguilar G, Huitron C (1993) Conidial and mycelial-bound exo-pectinase of Aspergillus sp. FEMS Microbiol Lett 108: 127–132
Bilang R, Iida S, Peterhans A, Potrykus I, Paszkowski J (1991) The 3′-terminal region of the hygromycin-resistance gene is important for its activity in Escherichia coli and Nicotiana tabacum. Gene 100: 247–250
Bryngelsson T, Gustaffsson M, Green B, Lind C (1988) Uptake of host DNA by the parasitic fungus Plasmodiophora brassicae. Physiol Mol Plant Pathol 33: 163–171
Feher Z, Schabik M, Kiss A, Zsindley A, Szabo G (1986) Characterization of inl+ transformants of Neurospora crassa obtained with a recombinant cosmid-pool. Curr Genet 11: 131–137
Fincham JRS (1989) Transformation in fungi. Microbiol Rev 53: 148–170
Gems D, Johnstone IL, Clutterbuck AJ (1991) An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency. Gene 98: 61–67
Golz C, Koehler F, Schieder O (1990) Transfer of hygromycin resistance into Brassica napus using total DNA of a transgenic B. nigra line. Plant Mol Biol 15: 475–483
Gupta V, Jagannathan V, Lakshmikumaran MS (1990) A novel AT rich tandem repeat of Brassica nigra. Plant Sci 68: 223–229
Jefferson RA (1987) Assaying chimeric genes in plants: the gus gene fusion system. Plant Mol Biol Rep 5: 387–405
Kellner M, Burmester A, Woestemeyer A, Woestemeyer J (1993) Transfer of genetic information from the mycoparasite Parasitella parasitica to its host Absidia glauca. Curr Genet 23: 334–337
Khanh NQ (1991) Transformation von Aspergillus niger mit Plasmiden. In: Bertram S, Gassen HG (eds) Gentechnische Methoden. Gustav Fischer Verlag, Stuttgart New York, pp 181–184
Lorenz MG, Wackernagel W (1993) Bacterial gene transfer in the environment. In: Wohrmann K, Tomiuk J (eds) Transgenic organisms. Advances in life science. Birkhauser Verlag, Basel, pp 43–64
Mehta A, Chopra S, Mehta P (1993) Production of cell-wall degrading enzymes by three isolates of Aspergillus niger under different cultivation conditions. J Basic Microbiol 33: 193–199
Mazodier P, Davies J (1991) Gene transfer between distantly related bacteria. Annu Rev Genet 25: 147–171
Pickardt T, Meixner M, Schade V, Schieder O (1991) Transformation of Vicia narbonensis via Agrobacterium-mediated gene transfer. Plant Cell Rep 9: 535–538
Punt PJ, Oliver RP, Dingemanse MA, Pouwels POH, Van den Hondel CAMJJ (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56: 117–124
Roberts IN, Oliver RP, Punt PJ, Van den Hondel CAMJJ (1989) Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi. Curr Genet 15: 177–180
Rogers SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5: 69–76
Romanowski G, Lorenz MG, Wackernagel W (1991) Adsorption of plasmid DNA to mineral surfaces and protection against DNase I. Appl Environm Microbiol 57: 1057–1061
Sacristan MD, Gerdemann-Knoerck M, Schieder O (1989) Incorporation of hygromycin resistance in Brassica nigra and its transfer to B. napus through asymmetric protoplast fusion. Theor Appl Genet 78: 194–200
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Schmidt-Rogge T, Meixner M, Srivastava V, Guha-Mukherjee S, Schieder O (1993) Transformation of haploid Datura innoxia protoplasts and analysis of the plasmid integration pattern in regenerated transgenic plants. Plant Cell Rep 12: 390–394
Stachel SE, Zambryski PC (1989) Generic trans-kingdom sex? Nature 340: 190–191
Stotzky G (1989) Gene transfer among bacteria in soil. In: Levy SB, Miller RV (eds) Gene transfer in the environment. McGraw-Hill, New York, pp 165–222
Stotzky G, Zeph LR, Devanas MA (1991) Physicochemical and biological factors affect the transfer of genetic information among microorganisms in soil. In: Ginzburg L (ed) Assessing ecological risks of biotechnology. Butterworth-Heinemann, Stoneham, Massachusetts, pp 95–122
Toepfer R, Proels M, Schell J, Steinbiss H-H (1987) A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Res 15: 7155–7174
Ward M, Wilson LJ, Kodama KH (1993) Use of Aspergillus over-producing mutants, cured for integrated plasmid, to overproduce heterologous proteins. Appl Microbiol Biotechnol 39: 738–743
Author information
Authors and Affiliations
Additional information
Communicated by K. Wolf
Rights and permissions
About this article
Cite this article
Hoffmann, T., Golz, C. & Schieder, O. Foreign DNA sequences are received by a wild-type strain of Aspergillus niger after co-culture with transgenic higher plants. Curr Genet 27, 70–76 (1994). https://doi.org/10.1007/BF00326581
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00326581