Abstract
The method of spreading transcription complexes has been applied to amphibian oocytes of Pleurodeles genus. Complexes of nucleolar origin show a regular and homogeneous organization similar to that described in other materials. The observations add to the interpretation as an amplification of nucleolar DNA and a redundancy of ribosomal cistrons in the two species studied. —On the other hand, complexes of chromosomal origin display a great diversity. Two main characteristics can be drawn: the existence of several transcription units in a chromosomal organization unit and the possibility to point out a special architecture at the RNP fibril level. Applying a shadowing technique used for isolated molecules is an improvement compared with earlier methods based on PTA coloration.
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Attachée de recherches C.N.R.S, Laboratoire de Biologie Animale, Orléans.
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Angelier, N., Lacroix, J.C. Complexes de transcription d'origines nucléolaire et chromosomique d'ovocytes de Pleurodeles waltlii et P. poireti (Amphibiens, Urodèles). Chromosoma 51, 323–335 (1975). https://doi.org/10.1007/BF00326319
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DOI: https://doi.org/10.1007/BF00326319