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Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe

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Abstract

Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen starvation and their induction occurred at several stages of spore formation. Analysis of the cDNA prmary structures revealed a capacity for the coding of polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively. Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect on spore formation.

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Author notes

  1. Shusei Sato & Satoshi Tabata

    Present address: Kazusa DNA Research Institute, 473-2 Okanazawa Midori-ku, 266, Chiba, Japan

Authors and Affiliations

  1. Department of Biology, School of Science, Nagoya University, Furoh-cho, Chikusa-ku, 464-01, Nagoya, Japan

    Shusei Sato, Utut Widyastuti, Yasuo Hotta & Satoshi Tabata

  2. Research Institute for Biological Sciences, Ajinomoto Co. LTD, Maeda-cho, Totsuka-ku, 244, Yokohama, Japan

    Hideki Suzuki

  3. Department of Biology, Faculty of Mathematics and Science, Bogor Agriculture Institute, Pajajaran, 16143, Bogor, Indonesia

    Utut Widyastuti

Authors
  1. Shusei Sato
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  2. Hideki Suzuki
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  3. Utut Widyastuti
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  4. Yasuo Hotta
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  5. Satoshi Tabata
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Communicated by M. Yamamoto

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Sato, S., Suzuki, H., Widyastuti, U. et al. Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe . Curr Genet 26, 31–37 (1994). https://doi.org/10.1007/BF00326301

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  • DOI: https://doi.org/10.1007/BF00326301

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