Abstract
The trypsin banding methods were applied to feather pulp and embryonic material of the chicken. Two contrasting types of chromosomal banding patterns were obtained by varying the duration of trypsin treatment. A short time treatment shows a G-banding pattern which has characteristic and distinctive bands along the chromosome arms. Prolonging the trypsin treatment causes the G-banding pattern to disappear, and only the centromeres and the W chromosome remained heterochromatic which is characteristic of the C-banding pattern. The application of the G-banding pattern analysis was used to identify regions of chromosomes involved in rearrangements. The simplified trypsin technique which produces the C-banding pattern makes it relatively easy to identify the W sex-chromosome and determine sex in avian species.
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Wang, N., Shoffner, R.N. Trypsin G- and C-banding for interchange analysis and sex identification in the chicken. Chromosoma 47, 61–69 (1974). https://doi.org/10.1007/BF00326271
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DOI: https://doi.org/10.1007/BF00326271