Summary
Pea chloroplast DNA and cloned restriction fragments of pea chloroplast DNA have been used as templates in a cell-free coupled transcription-translation system from E. coli and have been shown to direct the synthesis of a 39 kD polypeptide related to cytochrome f (37.3 kD). The product was shown to be similar to cytochrome f by immunoprecipitation with specific antibodies and by peptide mapping of the products of limited chymotrypsin digestion. The structural gene for this higher molecular weight form of cytochrome f has been located within a 3.3 kbp BglII fragment situated 18 kbp from the single 16S rRNA gene.
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Willey, D.L., Huttly, A.K., Phillips, A.L. et al. Localization of the gene for cytochrome f in pea chloroplast DNA. Molec Gen Genet 189, 85–89 (1983). https://doi.org/10.1007/BF00326059
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DOI: https://doi.org/10.1007/BF00326059