Summary
One histidine-requiring clone (PO3) of Hyoscyamus muticus was isolated by testing 375 surviving colonies in an experiment in which 5-bromodeoxyuridine (BUdR) was applied to cell cultures derived from N-methyl-N'-nitro-nitrosoguandine (MNNG) treated haploid leaf protoplasts. In subsequent experiments inconsistent effects of BUdr on cell growth were observed and no further auxotrophs were obtained. Growth of PO3 was shown to be proportional to histidine concentrations up to 0.5 mM and two presumptive histidine precursors, histidinol phosphate and histidinol, also supported growth. A preliminary analysis of PO3 by somatic hybridization using two other auxotrophic clones of H. muticus indicated that the histidine auxotrophy of PO3 is a recessive trait. Further, as PO3 does not complement to wildtype with another His- line, VA5, these two His- lines may have the same biochemical lesion. Spontaneous protrophic revertants were present at a frequency of 3×10-7 per viable cell and this frequency was increased ca. 60 fold by MNNG treatment. Four tested revertants grew equally well in the presence or absence of histidine.
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Communicated by G. Melchers
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Shimamoto, K., King, P.J. Isolation of a histidine auxotroph of Hyoscyamus muticus during attempts to apply BUdR enrichment. Molec Gen Genet 189, 69–72 (1983). https://doi.org/10.1007/BF00326056
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DOI: https://doi.org/10.1007/BF00326056