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Identification of two closely located larval salivary protein genes in Drosophila melanogaster

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Summary

One of the protein bands (sgs-3) resulting from urea gel electrophoresis of the larval saliva from a wild-type strain of Drosophila melanogaster, was shown to consist of two proteins of differing molecular weights. This was demonstrated by using two-dimensional gel electrophoresis combining urea with sodium dodecyl sulphate (SDS) gels. Genetic and cytogenetic analysis show that these proteins are determined by two independent genes that genetic evidence indicates are the structural genes for these proteins. Both genes are shown to be located close together at the 68C puff region on the left arm of chromosome three. The location of one of these genes corresponds to that of the gene for sgs-3 localised by Akam et al. (1978). The other gene is a hitherto unknown salivary protein gene that has been identified and localised in this study.

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Authors and Affiliations

  1. Molecular Biology Unit, Tata Institute of Fundamental Research, 400 005, Bombay, India

    N. Gautam

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  1. N. Gautam
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Communicated by Ch. Auerbach

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Gautam, N. Identification of two closely located larval salivary protein genes in Drosophila melanogaster . Molec. Gen. Genet. 189, 495–500 (1983). https://doi.org/10.1007/BF00325916

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  • DOI: https://doi.org/10.1007/BF00325916

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