Summary
Brief exposure of Daphnia pulex to 10% aqueous glucose solution induced the rapid disappearance of the electrophoretically normal fraction of G6PD and the appearance of a slow fraction. The mobility of the slow fraction was similar to that of the S-fraction spontaneously arising in the variable clones (Ruvinsky et al. 1983). Actinomycin D, α-amanitine and cycloheximide blocked glucose-induced changes in the EM of G6PD. Evidence was obtained for a factor, presumably a polypeptide, being synthesized de novo during the induction experiments. This factor is possibly capable of modifying the conformation of G6PD.
The spontaneously arising and induced changes in the EM of G6PD seem to have similar mechanisms. It is suggested that the activation of latent genetic material may be responsible for changes in the EM of G6PD.
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Communicated by H. Böhme
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Ruvinsky, A.O., Lobkov, Y.I. & Belyaev, D.K. Spontaneous and induced activation of genes affecting the phenotypic expression of glucose 6-phosphate dehydrogenase in Daphnia pulex . Molec. Gen. Genet. 189, 490–494 (1983). https://doi.org/10.1007/BF00325915
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DOI: https://doi.org/10.1007/BF00325915