Summary
The complete sequence of a sunflower (Helianthus annuus) gene, HaG5, encoding a 2 S albumin storage protein was determined. The predicted unprocessed precursor has 295 amino acids, is rich in glutamine residues (24%) and contains a hydrophobic amino-terminus that is similar to the consensus signal peptide. Amino acid sequencing of the mature protein revealed extensive post-translational processing. Nuclease protection and primer extension analysis indicated a major transcriptional start 30 nucleotides 5′ of the predicted ATG start codon. Additional sequence data, determined from a nearly full length cDNA recombinant, indicate that HaG5 is a member of a small gene family comprised of at least two divergent genes. Comparison of the predicted HaG5 gene product with sequences of other known plant proteins revealed distant but significant homology with the napins of Brassica and other heterogeneous seed proteins in the albumin superfamily.
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Aebersold RH, Teplow DB, Hood LE, Kent SBH (1986) High efficiency preparation of proteins from analytical sodium dodecyl sufate-polyacrylamide gels for direct sequence analysis. J Biol Chem 261:4229–4238
Allen RD, Nessler CL, Thomas TL (1985) Developmental expression of sunflower 11S storage protein genes. Plant Mol Biol 5:165–173
Allen RD, Cohen EA, Vonder Haar RA, Orth KA, Ma DP, Nessler CL, Thomas TL (1987) Expression of embryo specific genes in sunflower. In: Davidson EH, Firtel R (eds) UCLA symposium on molecular approaches to developmental biology. Alan R. Liss, New York, p 415
Ampe C, Van Damme J, deCastro LAB, Sampaio MJAM, Van Montagu M, Vandekerckhove J (1986) The amino-acid sequence of the 2S sulphur rich proteins from seeds of Brazil nut (Bertholletia excelsa H.B.K.). Eur J Biochem 159:597–604
Benton WD, Davis RW (1977) Screening λgt recombinant clones by hybridization to single plaques in situ. Science 196:180–182
Butterworth AG, Lord JM (1983) Ricin and Ricinus communis agglutin subunits are all derived from a single-size polypeptide precursor. Eur J Biochem 137:57–65
Campos FAP, Richardson M (1983) The complete amino acid sequence of the bifunctional α-amylase/trypsin inhibitor from seeds of ragi (Indian finger millet, Elusine coracana). FEBS Lett 152:300–304
Casey R, Domoney C, Ellis N (1986) Legume storage proteins and their genes. Oxford Surv Plant Mol Cell Biol 3:1–95
Cohen EA (1986) Analysis of sunflower 2S seed storage protein genes MS Thesis, Texas A&M University
Crouch ML, Tenbarge KM, Simon AE, Ferle R (1983) cDNA clones for Brassica napus seed storage proteins: evidence from nucleotide sequence analysis that both subunits of napin are cleaved from a single precursor polypeptide. J Mol Appl Genet 2:273–284
Cronquist A (1968) The evolution and classification of flowering plants. Houghton Mifflin, Boston, pp 127–131
Dale RMK, McClure BA, Houchins JP (1985) A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: Application to sequencing the corn mitochondrial 18S rDNA. Plamsid 13:31–40
Dalgalarrondo M, Raymond J, Azanza JL (1984) Sunflower seed proteins: characterization and subunit composition of the globulin fraction. J Exp Bot 35:1618–1628
Ericson ML, Rödin J, Lenman M, Glimelius K, Josefsson LG, Rask L (1986) Structure of the rapeseed 1.7S storage protein, napin, and its precursor. J Biol Chem 261:14576–14581
Favaloro J, Treisman R, Kamen R (1980) Transcription maps of polyoma virus specific RNA: Analysis by two-dimensional nuclease S 1 mapping. Methods Enzymol 65:718–749
Frishauf AM, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170:827–842
Forde J, Forde BG, Fry RP, Kreis M, Shewry PR, Miflin BJ (1983) Identification of barley and wheat cDNA clones related to the high Mr polypeptides of wheat gluten. FEBS Lett 162:360–366
Heidecker G, Messing J (1986) Structural analysis of plant genes. Annu Rev Plant Physiol 37:439–466
Higgins TJV (1984) Synthesis and regulation of major proteins in seeds. Annu Rev Plant Physiol 35:191–221
Higgins TJV, Chandler PM, Randall PJ, Spencer D, Beach LR, Blagrove RJ, Kort AA, Inglis AS (1986) Gene structure, protein structure and regulation of the synthesis of a sulfur-rich protein in pea seeds. J Biol Chem 261:11124–11130
Higgins TJV, Beach LR, Spencer D, Chandler PM, Randall PJ, Blagrove RJ, Kortt AA, Guthrie RE (1987) cDNA and protein sequence of a major pea seed albumin (PA2: Mr∼26000). Plant Mol Biol 8:37–45
Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ (1985a) Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat barley and rye. Oxford Surv Plant Mol Cell Biol 2:253–317
Kreis M, Forde BG, Rahman S, Miflin BJ, Shewry PR (1985b) Molecular evolution of seed storage proteins of barley, rye and wheat. J Mol Biol 183:499–502
Leach DRF, Stahl FW (1983) Viability of λ phage carrying a perfect palindrome in the absence of recombination nucleases. Nature 305:448–451
Lord JM (1985) Synthesis and intracellular transport of lectin and storage protein precursors in endosperm of castor bean. Eur J Biochem 146:403–409
Maeda K, Hase T, Matsubara H (1983) Complete amino acid sequence of an α-amylase inhibitor in wheat kernel. Biochim Biophys Acta 743:52–57
Mahoney WC, Hermodson MA, Jones B, Powers DD, Corfman RS, Reeck GR (1984) Amino acid sequence and secondary structural analysis of the corn inhibitor of trypsin and activated Hageman factor. J Biol Chem 259:8412–8416
Maniatis T, Jeffrey A, Kleid DG (1975) Nucleotide sequence of the rightward operator of phage λ. Proc Natl Acad Sci USA 72:1184–1189
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor
Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–78
Mount S (1982) A catalogue of splice junction sequences. Nucleic Acids Res 10:459–472
Odani S, Koide T, Ono T (1983) The complete amino acid sequence of barley trypsin inhibitor. J Biol Chem 258:7998–8003
Laroche-Raynal M, Delseny M (1986) Identification and characterization of the mRNA for major storage proteins from radish. Eur J Biochem 157:321–327
Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA (1980) Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143:161–178
Schwenke KD, Pahtz W, Linow KJ, Schultz M (1979) On oil seed proteins, part 11. Purification, chemical composition and some physico-chemical properties of the 11 S globulin (Helianthinin) in sunflower seed. Die Nahrung 23:241–254
Serfling E, Jasin M, Schaffner W (1985) Enhancers and eukaryotic transcription. Trends Genet 1:224–230
Sharief FS, Li SL (1982) Amino acid sequence of small and large subunits of seed storage proteins from Ricinus communis. J Biol Chem 257:14753–14759
Sun SSM, Altenbach SB, Leung FW (1987) Properties, biosynthesis and processing of a sulfur-rich protein in Brazil nut (Bertholletia excelsa H.B.K.). Eur J Biochem 162:477–483
Templeman TS, Demaggio AE, Stein DB (1986) An ostrich fern storage protein shares genetic homology with the 1.7S rapeseed storage protein. Am J Bot 73:688
von Heijne G (1986) A new method for predicting signal sequence cleavage. Nucleic Acids Res 14:4683–4690
Youle RJ, Huang AHC (1981) Occurrence of low molecular weight and high cysteine containing albumin storage proteins in oil seeds of diverse species. Am J Bot 68:44–48
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Communicated by R.B. Goldberg
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Allen, R.D., Cohen, E.A., Vonder Haar, R.A. et al. Sequence and expression of a gene encoding an albumin storage protein in sunflower. Molec Gen Genet 210, 211–218 (1987). https://doi.org/10.1007/BF00325686
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DOI: https://doi.org/10.1007/BF00325686