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A new non-enzymatic tracer for time-resolved fluoroimmunoassay of triazine herbicides

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  • Environmental Analysis
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Summary

The synthesis, characterization and application of a new kind of conjugate as tracer for use in time-resolved fluoroimmunoassay of triazine herbicides is described. Bovine serum albumin (BSA) served as carrier molecule, to which a triazine herbicide derivative and the Eu(III)-chelate W8044-Eu were subsequently coupled. The conjugate was characterized after each synthesis step by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The total composition was 1 BSA : 5.2 (±0.7) haptens : 5.1 (±0.4) fluorophores as calculated from the MALDI-MS data. The conjugate was successfully applied in competitive fluoroimmunoassays with sequential or simultaneous incubation of herbicide and tracer. With the sequential assay, the lowest detection limit was 0.1 μg/l for terbutryn. Results of assays performed with microtiter strips were compared with those obtained with commercial micro affinity columns.

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Authors and Affiliations

  1. Lehrstuhl für Analytische Chemie der Universität, Wilhelm-Klemm-Strasse 8, D-48149, Münster, Germany

    Monika Wortberg & Karl Cammann

  2. Institut für Chemo- und Biosensorik, Wilhelm-Klemm-Strasse 8, D-48149, Münster, Germany

    Karl Cammann

  3. Institut für Medizinische Physik und Biophysik der Universität, Robert-Koch-Strasse 31, D-48149, Münster, Germany

    Kerstin Strupat & Franz Hillenkamp

Authors
  1. Monika Wortberg
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  2. Karl Cammann
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  3. Kerstin Strupat
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  4. Franz Hillenkamp
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Wortberg, M., Cammann, K., Strupat, K. et al. A new non-enzymatic tracer for time-resolved fluoroimmunoassay of triazine herbicides. Fresenius J Anal Chem 348, 240–245 (1994). https://doi.org/10.1007/BF00325366

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  • DOI: https://doi.org/10.1007/BF00325366

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