Summary
Our previous radioautographic experiments suggesting absorption of lipid by the nucleus have been followed by direct examination of liver cell nuclei isolated from the animals at various times after administration of palmitic acid-9,10-3H. Twenty four hours post administration, the radioactivity in the isolated nuclei was still present in lipid. At this and all subsequent times examined, the total radioactivity in the nuclei did not exceed 1 percent of the total present in the liver. Treatment of the nuclei with Triton X100, to remove the nuclear membranes, led to the loss of most of the radioactivity; that remaining was associated primarily with the chromatin, with very little being found in the nucleolar fraction.
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This work was started before the death of Dr. Wassermann on June 16, 1969; this paper is respectfully dedicated to his memory.
Work supported by the U. S. Atomic Energy Commission.
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Barton, A.D., Kisieleski, W.E. & Wassermann, F. Concerning the uptake of lipid by the nucleus. Z. Zellforsch. 115, 307–313 (1971). https://doi.org/10.1007/BF00324933
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DOI: https://doi.org/10.1007/BF00324933