Summary
The distributions of 3H-putrescine and 3H-uridine were studied autoradiographically in cultured Xenopus laevis liver cells. Biochemical assay showed that at 4 h 10%, and at 24 h 30 % of the putrescine label was recovered as spermidine. Grain counts per unit surface area in light microscopic autoradiographs indicate that the 3H-polyamines show a similar intranuclear accumulation as 3H-uridine with a definite association with the nucleolus. The time course is different, however since 3H-polyamines continue to accumulate in the nucleus, while 3H-uridine reaches a peak nuclear concentration within 30 min and drops to one-half after 24 h. No instance of grains overlying mitotic figures was observed. These findings indicate an association of 3H-polyamines with nuclear and nucleolar RNA.
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References
Beaumont, A.: Sur la culture in vitro de foie larvaire d'Amphiben: apparition du glycogène en milieu synthétique. C. R. Acad. Sci. (Paris) 243, 676–677 (1956).
Brown, D. D.: RNA synthesis during early development. In: Developmental and metabolic control mechanisms in neoplasia, p. 219–236. Baltimore: Williams & Wilkins 1965.
Caldera, C. M., Barbiroli, B., Moruzzi, G.: Polyamines and nucleic acids during development of the chick embryo. Biochem. J. 97, 84–88 (1965).
Croisille, Y., LeDouarin, N. M.: Development and regeneration of the liver. In: Organogenesis (Ursprung and DeHaan, Ed.), p. 421–466. New York: Holt, Rinehart and Winston 1965.
Dion, A. S., Herbst, E. J.: The localization of spermidine in salivary gland cells of Drosophila melanogaster and its effect on 3H-uridine incorporation. Proc. nat. Acad. Sci. (Wash.) 58, 2367–2371 (1967).
Dykstra, W. G., Jr., Herbst, E. J.: Spermidine in regenerating liver: relation to rapid synthesis of ribonucleic acid. Science 149, 428–429 (1965).
Graham, A. F., Rake, A. V.: RNA synthesis and turnover in mammalian cells propagated in vitro. Ann. Rev. Microbiol. 17, 139–166 (1963).
Jänne, J.: Studies on the biosynthetic pathway of polyamines in rat liver. Acta physiol. scand., Suppl. 300, 1–71 (1967).
—, Raina, A.: Stimulation of spermidine synthesis in the regenerating rat liver: relation to increased ornithine decarboxylase activity. Acta chem. scand. 22, 1349–1351 (1968).
—: On the stimulation of ornithine decarboxylase and RNA polymerase activity in rat liver after treatment with growth hormone. Biochim. biophys. Acta (Amst.) 174, 769–772 (1969).
Namba, M., Hirose, M., Kishimoto, R.: Properties and identification of rat liver cells in long term cultivation. Acta Med. Okayama 22, 209–217 (1968).
Pegg, A. E., Williams-Ashman, H. G.: On the role of S-adenosylmethionine in the biosynthesis of spermidine by rat prostate. J. biol. Chem. 244, 682–693 (1969).
Prescott, D. M., Bender, M. A.: Synthesis of RNA and protein during mitosis in mammalian tissue culture cells. Exp. Cell Res. 26, 260–268 (1962).
Raina, A., Cohen, S. S.: Polyamines and RNA synthesis in a polyauxotrophic strain of E. coli. Proc. nat. Acad. Sci. (Wash.) 55, 1587–1593 (1966).
—, Jänne, J., Sümes, M.: Stimulation of polyamine synthesis in relation to nucleic acids in regenerating rat liver. Biochim. biophys. Acta (Amst.) 123, 197–201 (1966).
—, Telaranta, T.: Association of polyamines and RNA in isolated subcellular particles from rat liver. Biochim. biophys. Acta (Amst.) 138, 200–203 (1967).
Russell, D. H.: Polyamine synthesis in amphibian embryos. Fed. Proc. 29, 669 (1970a).
—: Elevated polyamine synthesis in a mouse L 1210 leukemia. Proc. Amer. Ass. Cancer Res. 11, 69 (1970b).
—: Discussion: Putrescine and Spermidine Biosynthesis in growth and development. Ann. N. Y. Acad. Sci. 171, 772–782 (1970c).
—, Snyder, S. H.: Amine synthesis in rapidly growing tissues: ornithine decarboxylase activity in regenerating rat liver, chick embryo and various tumors. Proc. nat. Acad. Sci. (Wash.) 60, 1420–1427 (1968).
—: Amine synthesis in regenerating rat liver: effect of hypophysectomy and growth hormone on ornithine decarboxylase. Endocrinology 84, 223–228 (1969).
Russell, D. H., Snyder, S. H.: Amine synthesis in regenerating rat liver: extremely rapid turnover of ornithine decarboxylase. Molec. Pharmacol. 5, 253–262 (1969).
—, Medina, V. J.: Growth hormone induction of ornithine decarboxylase in rat liver. Endocrinology 86, 1414–1419 (1970).
Snyder, S. H., Kreuz, D. S., Medina, V. J., Russell, D. H.: Polyamine synthesis and turnover in rapidly growing tissues. Ann. N. Y. Acad. Sci. 171, 749–771 (1970).
Tabor, H., Tabor, C. W.: Spermidine, spermine and related amines. Pharmacol. Rev. 16, 245–300 (1964).
Weibel, E. R., Kistler, G. S., Scherle, W. F.: Practical sterological methods for morphometric cytology. J. Cell Biol. 30, 23–38 (1966).
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Supported by US-PHS Grant No. NS-07934
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Gfeller, E., Russell, D.H. Autoradiographic distributions of 3H-putrescine and 3H-uridine in a Xenopus liver cell line. Z. Zellforsch. 120, 321–331 (1971). https://doi.org/10.1007/BF00324895
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DOI: https://doi.org/10.1007/BF00324895