Abstract
Male Wistar rats exposed to 50, 100 or 300 ppm methyl tertiary-butyl ether vapour for 2–15 weeks, 6 h daily, 5 days a week, showed a dose-dependent blood ether concentration after 2 weeks' exposure. Blood concentrations of teriary-butanol, were also dose dependent indicating metabolic breakdown of the ether in vivo. The blood ether concentrations decreased after 6 weeks of exposure at the 50 ppm dose level and remained unaffected at higher doses while tertiary-butanol concentrations increased after 6 weeks with all doses, and began to decrease thereafter. Exposure caused a transient increase in UDP-glucuronosyltransferase activities in liver and kidney microsomes, almost no effects on hepatic cytochrome P-450 concentrations and a minor induction of kidney microsomal cytochrome P-450 content. Exposure produced almost no effect on brain succinate dehydrogenase, creatine kinase or acetylcholinesterase activities, while early inhibition of muscle creatine kinase activity was noted, accompanied by increased activity at the end of exposure.
References
Aitio A (1978) A simple and sensitive assay for the ethoxycoumar-in deethylation. Anal Biochem 85: 488–491
Brown NA, Holt D, Webb M (1984) The teratogenicity of methoxyacetic acid in the rat. Toxicol Lett 22: 93–100
Cederbaum AI, Cohen G (1980) Oxidative demethylation of t-butyl alcohol by rat liver microsomes. Biochem Biophys Res Commun 97: 730–736
Cinti DL, Moldeus P, Schenkman JB (1972) Kinetic parameters of drug-metabolizing enzymes in Ca2+C-sedimented microsomes from rat liver. Biochem Pharmacol 21: 3249–3256
Eells JT, Black KA, Makar AB, Tedford CE, Tephly TR (1982) The regulation of one-carbon oxidation in the rat by nitrous oxide and methionine. Arch Biochem Biophys 219: 316–326
Elliott TH, Parke DV, Williams RT (1959) Studies in detoxication. 79. The metabolism of cyclo (14C)hexane and its derivatives. Biochem J 72: 193–200
Hänninen O (1968) On the metabolic regulation in the glucuronic pathways in the rat tissues. Ann Acad Sci Fenn (Med) 142: 1–96
Lim R, Hsu L-W (1971) Studies on brain-specific membrane proteins. Biochim Biophys Acta 249: 569–582
McComb JA, Goldstein DB (1979) Quantitative comparison of physical dependence on tertiary butanol and ethanol in mice: Correlation with lipid solubility. J Pharmacol Exp Ther 208: 113–117
Omura T, Sato R (1964) The carbon monoxide-binding pigment of liver microsomes. II. Solubilization, purification and properties. J Biol Chem 239: 2379–2385
Phillips A, Langdon RG (1962) Hepatic triphosphopyridine nucleotidecytochrome c reductase: Isolation, characterization and kinetic studies. J Biol Chem 237: 2652–2660
Raichle ME, Eichung JO, Straatmann MG, Welch MJ, Larson KB, Ter-Pogossian MM (1976) Blood-brain barrier permeability of 11-labeled alcohols 15O-labeled water. Am J Physiol 230: 543–552
Savolainen H (1980) Glial cell toxicity of ethyleneglycol monomethylether vapor. Environ Res 22: 423–430
Stenger E-G, Aeppli L, Müller D, Peheim E, Thomann P (1971) Zur Toxikologie des Äthylenglykol-Monoäthyläthers. Arzneim-Forsch 21: 880–885
Stowell A, Aune H, Mørland J (1981) Production of acetaldehyde and ethanol by isolated rat liver parenchymal cells in the presence of diethyl ether. Biochem Pharmacol 30: 1967–1972
Szasz G, Gruber W (1978) Creatine kinase in serum: 4. Differences in substrate affinity among the isoenzymes. Clin Chem 24: 245–249
Wallimann T, Doetschman TC, Eppenberger HM (1983) Novel staining pattern of skeletal muscle M-lines upon incubation with antibodies against MM-creatine kinase. J Cell Biol 96: 1772–1779
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Savolainen, H., Pfäffli, P. & Elovaara, E. Biochemical effects of methyl tertiary-butyl ether in extended vapour exposure of rats. Arch Toxicol 57, 285–288 (1985). https://doi.org/10.1007/BF00324794
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DOI: https://doi.org/10.1007/BF00324794