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Changes in the excretion of endogenous glycine conjugate as a possible artifact in toxicological experiments

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Abstract

Changes in the urinary excretion of hippuric acid (HIA) and phenaceturic acid (PUA) as well as their metabolic precursors, i.e. benzoic (BA) and phenylacetic acid (PAA), in rats housed in glass metabolic cages for 4 days were monitored using gas-liquid chromatography. The amount of HIA excreted was 128±63 μmol/kg for female and 79±43 μmol/kg for male rats in the first 24 h and decreased to 11±7 μmol/kg (p< 0.01) for female and 3.2±2.4 μmol/kg (p< 0.001) for male rats on the 2nd day. These values remained nearly at the same level until the end of the experiment. The amount of PUA decreased from 48±12 μmol/kg on the 1st day to 22±9 μmol/kg (p< 0.05) on the 2nd day by male rats, whereas by the females the decrease from 30±9 μmol/kg to 21±8 μmol/kg was not significant. The decrease in the excretion of glycine conjugates was compensated by a parallel increase in the level of unconjugated BA and PAA.

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References

  • Adamson RH, Bridges JW, Evans ME, Williams RT (1970) Species differences in the aromatization of quinic acid in vivo and the role of gut bacteria. Biochem J 116: 437–443

    Google Scholar 

  • Asatoor AM (1965) Aromatization of quinic acid and shikimic acid by bacteria and the production of urinary hippurate. Biochim Biophys Acta 100: 290–292

    Google Scholar 

  • Bernhard K, Vuilleumier JP, Brubacher G (1955) The formation of benzoic acid in the animal body. Helv Chim Acta 38: 1438–1444

    Google Scholar 

  • Booth AN, Robbins DJ, Masri MS, De Eds F (1960) Excretion of catechol after ingestion of quinic and shikimic acids. Nature 187: 691

    Google Scholar 

  • Chantrenne H (1951) the requirement for coenzyme A in the enzymatic synthesis of hippuric acid. J Biol Chem 189: 227–233

    Google Scholar 

  • Curtius HCh, Völlmin JA, Baerlacher K (1972) The use of deuterated phenylalanine for the elucidation of the phenylalanine — tyrosine metabolism. Clin Chim Acta 37: 277–285

    Google Scholar 

  • Geradze LN (1979) Some biochemical shifts in the bodies of white rats after phthalan administration. Sb. Tr. NII Gigieny Truda i Profzabolev. GruzSSR 17: 186–189 Chem Abstr 94: 186 569 m

    Google Scholar 

  • Geradze LN (1982) Remote sequels in the long-term effect of phthalan. Vrach. Delo 7: 106–108, Chem. Abstr. 97: 139 908 y

    Google Scholar 

  • Gorkavi PG, Stepanov NG, Ulanova IP, Samoilova LM (1966) Disturbance of synthesis of hippuric acid in white rats in intoxications caused by chemicals used in industry. Toksikol Novykh Prom Khim Veshchestv 8: 5–14, Chem Abstr 72: 102 574 r

    Google Scholar 

  • James MO, Smith RL, Williams RT, Reidenberg M (1972) Conjugation of phenylacetic acid in man, subhuman primates and some non-primate species. Proc R Soc Lond, Series B 182: 25–35

    Google Scholar 

  • Jones AR (1982) Some observation on the urinary excretion of glycine conjugates by laboratory animals. Xenobiotica 12: 387–395

    Google Scholar 

  • Loseva IE (1969) Toxicological appraisal of methyl methacrylate. Tr Azerb Nauch-Issled Inst Gig Tr Prof Zabol 4: 105–109, Chem Abstr 76: 81 869 j

    Google Scholar 

  • Moldave K, Meister A (1957) Enzymatic acylation of glutamine by phenylacetic acid. Biochim Biophys Acta 24: 654–655

    Google Scholar 

  • Nandi DL, Lucas SV, Webster Jr. LT (1979) Benzoyl-coenzyme A: glycine N-acetyltransferase from bovine liver mitochondria. Purification and characterization. J Biol Chem 254: 7230–7234

    Google Scholar 

  • Ohtsuji H, Ikeda M (1971) Phenobarbital-induced protection against toxicity of toluene and benzene in the rat. Toxicol Appl Pharmacol 20: 30–43

    Google Scholar 

  • Rowe VD, Fales HM, Pisano JJ, Andersen AE, Guraff G (1975) Urinary metabolites of phenylalanine in the preweating rat treated with p-chlorophenylalanine. Biochem Med 12: 123–136

    Google Scholar 

  • Schachter D, Taggart JV (1953) Benzoyl coenzyme A and hippurate synthesis. J Biol Chem 203: 925–934

    Google Scholar 

  • Seutter-Berlage F, van Dorp HL, Kosse HGJ, Henderson PTh (1977) Urinary mercapturic acid excretion as a biological parameter of exposure to alkylating agents. Int Arch Occup Environ Health 39: 45–51

    Google Scholar 

  • Teuchy H, Quatacker J, Wolf G, van Sumere CF (1971) Quantitative investigation of hippuric acid formation in the rat after administration of some possible aromatic and hydroaromatic precursors. Arch Int Physiol Biochim 79: 573–587

    Google Scholar 

  • Thabrew MJ, Olorungsogo OO, Olowookere JO, Bababunmi EA (1982) Possible defect in xenobiotic activation before glycine conjugation in protein-energy malnutrition. Xenobiotica 12: 849–853

    Google Scholar 

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Authors and Affiliations

  1. Institute of Hygiene and Epidemiology, CS-10042, Prague, Czechoslovakia

    Igor Linhart & Jaroslav Smejkal

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  1. Igor Linhart
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  2. Jaroslav Smejkal
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Linhart, I., Smejkal, J. Changes in the excretion of endogenous glycine conjugate as a possible artifact in toxicological experiments. Arch Toxicol 61, 83–85 (1987). https://doi.org/10.1007/BF00324554

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  • DOI: https://doi.org/10.1007/BF00324554

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