Abstract
The effects of selenium on cellular toxicity and histochemical distribution of silver were examined in a cell culture system of mouse peritoneal macrophages. Selenium caused a significant delay in the appearance of coagulation necrosis induced by high silver concentrations and reduced the cytostatic effect of lower doses of silver when long-term toxicity was examined. Furthermore, selenium increased the amount of silver that could be visualized by autometallography. The additional silver made available for this histochemical demonstration was located in the cytosol as well as in lysosomes, the sole localization of silver when selenium was not administered.
Similar content being viewed by others
References
Aaseth J, Olsen A, Halse J, Hovig T (1981) Argyria-tissue deposition of silver as selenide. Scand J Clin Lab Invest 41: 247–251
Alexander J, Aaseth J (1981) Hepatobiliary transport and organ distribution of silver in the rat as influenced by selenite. Toxicology 21: 179–186
Baatrup E, Nielsen MG, Danscher G (1986) Histochemical demonstration of two mercury pools in trout tissues: mercury in kidney and liver after mercuric chloride exposure. Ecotoxicol Environ Saf: 267–282
Berry JP, Galle P (1982) Selenium and kidney deposits in experimental argyria. Electron microscopy and microanalysis. Pathol Biol 30: 136–140
Black RS, Whanger PD, Tripp MJ (1979) Influence of silver, mercury, lead, cadmium and selenium on glutathione peroxidase and transferase activities in rats. Biol Trace Elem Res 1: 313–324
Bleehen SS, Gould DJ, Harrington CI, Durrant TE, Slater DN, Underwood JCE (1981) Occupational argyria; light and electron microscopic studies and X-ray microanalysis. Br J Dermatol 104: 19–26
Cabe PA, Carmichael NG, Tilson HA (1979) Effects of selenium, alone and in combination with silver or arsenic, in rats. Neurobehav Toxicol 1: 275–278
Danscher G (1981) Light and electron microscopic localisation of silver in biological tissue. Histochemistry 71: 177–186
Danscher G (1982) Exogenous selenium in the brain. Histochemistry 76: 281–293
Danscher G (1984) Autometallography. Histochemistry 81: 331–335
Danscher G, Møller-Madsen B (1985) Silver amplification of mercury sulfide and selenide. J Histochem Cytochem 33: 219–228
Danscher G, Rungby J (1986) Differentiation of histochemically visualized mercury and silver. Histochem J 18: 109–114
Duckett S, Galle P, Fiori C (1985) Electron probe microanalysis of normal and pathological neural tissue with wavelenght dispersive X-ray spectrometry. In: Gabay S, Harris J, Ho BT (eds) Metal ions in neurology and psychiatry. Alan R Liss, NY
Ellermann-Eriksen S, Rungby J, Mogensen SC (1987) Autointerference of accumulation of silver in macrophages without affection of phagocytic, migratory and interferon-producing capacity. Virchows Archiv B, Cell Pathology (in press)
Ganther HE (1980) Interactions of vitamin E and selenium with mercury and silver. Ann NY Acad Sci 355: 212–226
Jensen LS (1975) Modification of a selenium toxicity in chicks by dietary silver and copper. J Nutr 105: 769–775
Jensen LS, Peterson RP, Falen L (1974) Inducement of enlarged hearts and muscular dystrophy in turkey poults with dietary silver. Poultry Sci 53: 57–64
Leirskar J (1974) On the mechanisms of cytotoxicity of silver and copper amalgams in a cell culture system. Scand J Dent Res 82: 74–81
Matuk Y (1983) Distribution of radioactive silver in the subcellular fractions of various tissues of the rat and its binding to low molecular weight proteins. Can J Physiol Pharmacol 61: 1391–1395
McDowell LR, Froseth JA, Piper RC (1978) Influence of arsenic, sulfur, cadmium tellurium, silver and selenium on the selenium-vitamin E deficiency in the pig. Nutr Rep Int 17: 19–33
Rahim AGA (1985) The effects of dietary L-ascorbic acid on the absorption and utilization of NaSeO3 of silver-treated rats. Comp Biochem Physiol 81C: 131–132
Rungby J (1986a) Exogenous silver in dorsal root ganglia, peripheral nerve, enteric ganglia and adrenal medulla. Acta Neuropathol 69: 45–53
Rungby J (1986b) Experimental argyrosis: ultrastructural localization of silver in rat eye. Exp Mol Pathol 45: 22–30
Rungby J, Danscher G (1983a) Localization of exogenous silver in brain and spinal cord of silver exposed rats. Acta Neuropathol 60: 92–98
Rungby J, Danscher G (1983b) Neuronal accumulation of silver in brains of progeny from argyric rats. Acta Neuropathol 61: 258–262
Rungby J (1987) Silver-induced lipid peroxidation: interactions with selenium and nickel. Toxicology (in press)
Rungby J, Hultman P, Ellermann-Eriksen S (1987) Silver affects viability and structure of cultured mouse peritoneal macrophages and peroxidative capacity of whole mouse liver. Arch Toxicol (in press)
Tanaka T, Hagashi Y, Ishizawa M (1983) Subcellular distribution and binding of heavy metals in the untreated liver of the squid, comparison with data from the livers of cadmium and silver exposed rats. Experientia 39: 746–748
Thorlacius-Ussing O, Graabæk PM (1986) Simultaneous ultrastructural demonstration of heavy metals (silver, mercury) and acid phosphatase. Histochem J 18: 639–646
Thorlacius-Ussing O, Rungby J (1984) Ultrastructural localization of exogenous silver in the anterior pituitary gland of the rat. Exp Mol Pathol 41: 58–66
Van Vleet JF, Boon GD, Ferrans VJ (1981) Induction of lesions of selenium-vitamin E deficiency in ducklings fed silver, copper, cobalt, tellurium, cadmium, or zinc: Protection by selenium or vitamin E supplements. Am J Vet Res 42: 1206–1217
Wagner PA, Hoekstra W, Ganther H (1975) Alleviation of silver toxicity by selenite in the rat in relation to tissue glutathione peroxidase. Proc Soc Exp Biol Med 148: 1106–1110
Yoshida M, Tashiro H, Iwami K, Yasumoto K, Iwai K (1983) Bioavailability of selenite, selenomethionine and selenocysteine in rats with silver loading. Agric Biol Chem 47: 807–813
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rungby, J., Ellermann-Eriksen, S. & Danscher, G. Effects of selenium on toxicity and ultrastructural localization of silver in cultured macrophages. Arch Toxicol 61, 40–45 (1987). https://doi.org/10.1007/BF00324546
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00324546