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Deletion-mapping of resistance against chlorate in Klebsiella aerogenes

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Summary

Chlorate-resistant mutants with deletions have been isolated from Klebsiella aerogenes. Mutants with deletions in the gal-bio region of the genetic map can be divided into 9 groups. From the properties of the various groups of mutants the gene order nic A-aro G-gal-chl D-hut-bio-uvr B-chl A is deduced. Furthermore deletions have been observed in a segment of the chromosome containing nic B, thi B, inl B, and chl G. Thi B and chl G are adjacent but no more information about the gene order in this segment can be given. In both segments a great homology is observed with the corresponding regions of the genetic maps of E. coli and S. typhimurium. Deletions of chl A, chl D or chl G have a pleiotropic effect. Mutants with a deletion of one of these genes do not produce nitrite from nitrate or gas from glucose.

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Authors and Affiliations

  1. Microbiology Department, Biological Laboratory, Free University, de Boelelaan 1087, Amsterdam, The Netherlands

    A. H. Stouthamer & K. Pietersma

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  1. A. H. Stouthamer
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  2. K. Pietersma
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Communicated by P. Starlinger

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Stouthamer, A.H., Pietersma, K. Deletion-mapping of resistance against chlorate in Klebsiella aerogenes . Molec. Gen. Genet. 106, 174–179 (1970). https://doi.org/10.1007/BF00323836

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  • DOI: https://doi.org/10.1007/BF00323836

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