Skip to main content
SpringerLink
Log in

Evaluation of the proteolysis degree with the o-phthalaldehyde/N-acetyl-L-cysteine reagent

  • Original Papers
  • Biological Materials
  • Published:
Fresenius' Journal of Analytical Chemistry Aims and scope Submit manuscript

Summary

The o-phthalaldehyde/N-acetyl-L-cysteine (OPA-NAC) reagent is applied to the spectrophotometric evaluation of the proteolytic activity of enzymes. The high stability of the OPA-NAC isoindoles makes a strict control of the time of reaction unnecessary. A mathematical expression is proposed to calculate proteolysis degrees, where the absorbance decrease of the OPA-NAC derivative of the protein itself during the hydrolysis process is taken into account. The method is applied to bovine serum albumine, caseine, lysozyme, lactoglobuline and protamine sulphate as substrates, and pronase, papaine, trypsin and chymotrypsin as enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ohta Y, Nakamura H, Samejima T (1972) J Biochem 72:521–527

    Google Scholar 

  2. Holmquist B, Vallee BL (1974) J Biol Chem 249:4601–4607

    Google Scholar 

  3. Reimerdes EH, Klostermeyer A (1976) Meth Enzymol 45:26–28

    Google Scholar 

  4. Bensadoun A, Weinstein D (1976) Anal Biochem 70:241–250

    Google Scholar 

  5. Binkley F, Leibach F, King N (1968) Arch Biochem 128:397–405

    Google Scholar 

  6. Lin Y, Means GE, Feeney RE (1969) J Biol Chem 244:789–792

    Google Scholar 

  7. Chism GW, Huang AE, Marshall JA (1979) J Dairy Sci 62:1798–1800

    Google Scholar 

  8. Pearce KN (1979) J Dairy Sci Technol 14:233–239

    Google Scholar 

  9. Beeby R (1980) J Dairy Sci Technol 15:99–108

    Google Scholar 

  10. García Alvarez-Coque MC, Medina Hernández MJ, Villanueva Camañas RM, Mongay Fernández C (1989) Anal Biochem 178:1–7

    Google Scholar 

  11. Taylor S, Tappel AL (1973) Anal Biochem 56:140–148

    Google Scholar 

  12. Porter DH, Swaisgood HE, Catignani GL (1982) Anal Biochem 123:41–48

    Google Scholar 

  13. Rowlett R, Murphy J. (1981) Anal Biochem 112:163–169

    Google Scholar 

  14. Church FC, Swaisgood HE, Porter DH, Catignani, GL (1983) J Dairy Sci 66:1219–1227

    Google Scholar 

  15. Church FC, Porter DH, Catignani GL, Swaisgood HE (1985) Anal Biochem 146:343–348

    Google Scholar 

  16. García Alvarez-Coque MC, Medina Hernández MJ, Villanueva Camañas RM, Mongay Fernández C (1989) Anal Biochem 180:172–176

    Google Scholar 

  17. Medina Hernández MJ, Villanueva Camañas RM, Monfort Cuenca E, García Alvarez-Coque MC (1990) The Analyst (in press)

  18. Goodno CC, Swaisgood HE, Catignani GL (1981) Anal Biochem 115:203–211

    Google Scholar 

  19. Fasman GD (ed) (1976) Handbook of biochemistry and molecular biology, vol III, 3rd edn, CRC Press, Ohio

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Química Analítica, Facultad de Química, Universitat de Valencia, Burjasot, E-46100, Valencia, Spain

    M. J. Medina Hernández, E. Bonet Domingo, R. M. Villanueva Camañas & M. C. García Alvarez-Coque

Authors
  1. M. J. Medina Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Bonet Domingo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. M. Villanueva Camañas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. C. García Alvarez-Coque
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hernández, M.J.M., Domingo, E.B., Camañas, R.M.V. et al. Evaluation of the proteolysis degree with the o-phthalaldehyde/N-acetyl-L-cysteine reagent. Fresenius J Anal Chem 338, 62–65 (1990). https://doi.org/10.1007/BF00322787

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00322787

Keywords

Search

Navigation