Skip to main content
SpringerLink
Log in

Determination of polyamines in biological materials by HPLC with 9-fluorenylmethyl chloroformate precolumn derivatization

  • Original Papers
  • Biological/Pharmaceutical Analysis
  • Published:
Fresenius' Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

Di- and polyamines have been determined in the femtomol range by liquid chromatography on a reversed-phase C8 column with a linear gradient of acetate buffer/acetonitrile as mobile phase and fluorimetric detection at 265/310 nm after simple derivatization with 9-fluorenylmethyl chloroformate (FMOC) within 3 min at pH 7.8 in presence of aspartic acid to remove excess reagent. The FMOC—polyamine-derivatives have been stable in a methanol—water-solution for at least 10 h. The method can be directly applied to crude plant extracts, and it is not interfered by carbohydrates and phenolics.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Galston AW (1983) Bioscience 33:382–388

    Google Scholar 

  2. Bagni A, Fracassini DS, Torrigiani P (1982) In: Wareing PF (ed) Plant growth substances. Academic Press, London, pp 473–482

    Google Scholar 

  3. Slocum RD, Kaur-Sawhney R, Galston AW (1984) Arch Biochem Biophys 235:283–303

    Google Scholar 

  4. Galston AW, Kaur-Sawhney R (1987) In: Davies PJ (ed) Plant hormones and their role in plant growth and development. Martinus Nijhoff, Dordrecht, pp 280–295

    Google Scholar 

  5. Drolet G, Dumbroff EB, Legge RL, Thompson JE (1986) Phytochemistry 25:367–371

    Google Scholar 

  6. Hiatt A, Malmberg RL (1988) Plant Physiol 86:441–446

    Google Scholar 

  7. Turner LB, Stewart GR (1986) J Exp Botany 37:170–177

    Google Scholar 

  8. Tenter M, Wild A (1991) J Plant Physiol 137:647–654

    Google Scholar 

  9. Schulz H, Huhn G, Härtling S (1994) In: Hüttl RF (ed) UmweltWissenschaften. Eberhard Blottner, Taunusstein (in press)

    Google Scholar 

  10. Seiler N (1980) In: Gaugas JM (ed) Polyamines in biomedical research. Wiley, New York, pp 435–461

    Google Scholar 

  11. Seiler N, Knodgen B (1978) J Chromatogr 145:29–39

    Google Scholar 

  12. Flores HE, Galston AW (1982) Plant Physiol 69:701–706

    Google Scholar 

  13. Skaaden T, Greibrokk T (1982) J Chromatogr 247:111–119

    Google Scholar 

  14. Einarsson S, Josefsson B, Lagerkvist S (1983) J Chromatogr 282:609–618

    Google Scholar 

  15. Godel H (1986) Thesis, University of Hohenheim, Stuttgart

  16. Gustavsson B, Betnér I (1990) J Crhomatogr 507:67–77

    Google Scholar 

  17. Hauschild MZ (1993) J Chromatogr 630:397–401

    Google Scholar 

  18. Villanueva VR, Mardon M, Le Goff MTh, Moncelon F (1987) J Chromatogr 393:97–105

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Ecotoxicology, Centre for Environmental Research Leipzig-Halle, P.O. Box 2, D-04301, Leipzig, Germany

    Gernot Huhn & Horst Schulz

  2. Department of Analytical Chemistry, Centre for Environmental Research Leipzig-Halle, P.O. Box 2, D-04301, Leipzig, Germany

    Jürgen Mattusch

Authors
  1. Gernot Huhn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jürgen Mattusch
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Horst Schulz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huhn, G., Mattusch, J. & Schulz, H. Determination of polyamines in biological materials by HPLC with 9-fluorenylmethyl chloroformate precolumn derivatization. Fresenius J Anal Chem 351, 563–566 (1995). https://doi.org/10.1007/BF00322733

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00322733

Keywords

Search

Navigation