Summary
The ultrastructure of the secondary lamellae of the gills and especially that of the “chloride cells” of Carassius aureus was studied. We found an amorphous, flakey, slightly adielectronic material in the areas of the “apical pits”. In order to determine the nature of this material, we studied these structures electronmicroscopically applying the periodic acid silver methenamine, colloidal iron and alcian blue methods. The periodic acid silver methenamine reaction, resulted in finely dispersed precipitations which were deposited in the areas of the “apical pits” and which correspond to the flakey material seen in the ordinary electron micrographs. The alcian blue method reveales strongly stained particles which form a more or less continuous film on the free surface of the lamellae, interrupted only at the level of the “chloride cells”. In these areas, notably within the “apical pits”, a rather thick layer of finely granular low-density material is attached to the plasma membrane. In taking into account other studies performed on this subject, as well as our own observations, we consider the material found on the surface of the “chloride cells” and particularly within their “apical pits” to be predominantly of glycoproteinous nature.
Résumé
L'ultrastructure des lamelles branchiales et spécialement celle des ≪chloride cells≫ du poisson rouge (Carassius aureus) a été étudiée. Nous avons constaté que du matériel amorphe floconneux, faiblement adiélectronique était attaché aux endroits des ≪creux apicaux≫. Afin de préciser la nature de ce matériel, nous avons étudié ces structures au microscope électronique avec les techniques suivantes: acide periodique méthènamine d'argent, colorations au fer colloïdal et au bleu d'alcian. Après la réaction à l'acide periodique méthènamine d'argent, de fines précipitations aux endroits des ≪creux apicaux≫, correspondant au matériel floconneux visible après la fixation au glutaraldéhyde tétroxyde d'osmium, étaient visibles. La coloration au bleu d'alcian révélait des particules fortement colorées formant un film plus ou moins continu à la surface libre des lamelles, sauf aux endroits oò les ≪chloride cells≫ sont en contact avec la surface. Là et notamment dans les 2čreux apicaux≫, du matériel légèrement granuleux, de faible densité, faisait une couche assez épaisse attachée à la membrane cellulaire. Tenant compte des résultats d'autres auteurs et de nos propres observations, nous considérons que la plus grande partie du matériel se trouvant à la surface des ≪chloride cells≫, et particulièrement dans les ≪creux apicaux≫, est de type glycoprotéique.
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Dédié à Monsieur le Professeur Dr Ernst Horstmann, Hambourg, à l'occasion de son soixantième anniversaire.
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Petřík, P., Bucher, O. A propos des ≪chloride cells≫ dans l'épithélium des lamelles branchiales du poisson rouge. Z. Zellforsch. 96, 66–74 (1969). https://doi.org/10.1007/BF00321478
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DOI: https://doi.org/10.1007/BF00321478