Abstract
Metaphase examination of pseudodiploid Chinese hamster cells revealed that spontaneous breaks or fission occurred rather frequently (2.9%) at the centromeric regions of subtelo- or metacentric chromosomes, resulting in the production of telocentric chromosomes. The centromeric fission appeared to occur in every member of the chromosome complement. An attempt was made to isolate cells possessing thus derived telocentrics from the cell population and gave two clonal lines which were retaining one and two telocentric chromosomes, respectively. Both banding and labeling patterns of these chromosomes indicated unequivocally their X chromosome origin. They were transmitted successively to the daughter cells during a 3-month culture period, showing no tendency to fuse to produce a metacentric chromosome.
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Brinkley, B. R., Stubblefield, E.: Ultrastructure and interaction of the kinetochore and centrioles in mitosis and meiosis. In: Advanc. Cell Biol. (D. M. Prescott, L. Goldstein and E. McConkey, eds.), vol. I, p. 119–185. Amsterdam: North-Holland Publishing Co. 1970.
Cole, A., Corry, P. M., Langley, R.: Effects of radiation and other agents on the molecular structure and organization of the chromosome. 23rd Ann. Symp. Found. Cancer Res., p. 346–379. Baltimore: Williams & Wilkins, Co. 1970.
Comings, D. E., Okada, T. A.: Whole-mount electron microscopy of the centromere region of metacentric and telocentric mammalian chromosomes. Cytogenetics 9, 436–449 (1970).
Ford, D. K., Yerganian, G.: Observations on the chromosomes of Chinese hamster cells in tissue culture. J. nat. Cancer Inst. 21, 393–425 (1958).
John, B., Hewitt, G. M.: Karyotype stability and DNA variability in the Acrididae. Chromosoma (Berl.) 20, 155–172 (1966).
John, B., Hewitt, G. M.: Patterns and pathways of chromosome evolution within the Orthoptera. Chromosoma (Berl.) 25, 40–74 (1968).
Kato, H., Sandberg, A. A.: Chromosome pulverization in human cells with micronuclei. J. nat. Cancer Inst. 40, 165–179 (1968).
Kato, H., Yosida, T. H.: Isolation of aneusomic clones from Chinese hamster cell line following induction of nondisjunction. Cytogenetics 10, 392–403 (1971).
Kato, H., Yosida, T. H.: Differential responses of several aneusomic cell clones to ultra-violet irradiation. Exp. Cell Res. (in press, 1972).
Kato, R.: Localization of “spontaneous” and Rous sarcoma virus-induced breakage in specific regions of the chromosomes of the Chinese hamster. Hereditas (Lund) 58, 221–247 (1967).
Lima-de-Faria, A.: The role of the kinetochore in chromosome organization. Hereditas (Lund) 42, 85–160 (1956).
Nadler, C. F., Hoffmann, R. S., Greer, K. R.: Chromosomal divergence during evolution of ground squirrel populations (Rodentia: Spermophilus). Syst. Zool. 20, 298–305 (1971).
Nadler, C. F., Hoffmann, R. S., Pizzimenti, J. J.: Chromosomes and serum proteins of prairie dogs and a model of Cynomys evolution. J. Mammalogy 52, 545–555 (1971).
Southern, D. I.: Stable telocentric chromosomes produced following centric misdivision in Myrmeleotettix maculatus (Thunb.). Chromosoma (Berl.) 26, 140–147 (1969).
Todd, N. B.: Karyotypic fissioning and canid phylogeny. J. theor. Biol. 26, 445–480 (1970).
Wahrman, J., Goitein, R., Nevo, E.: Mole rat Spalax: Evolutionary significance of chromosome variation. Science 164, 82–84 (1969).
White, M. J. D.: Chromosomal rearrangements and speciation in animals. Ann. Rev. Genet. 3, 75–98 (1969).
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Contribution No. 897 from the National Institute of Genetics, Japan.
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Kato, H., Sagai, T. & Yosida, T.H. Stable telocentric chromosomes produced by centric fission in chinese hamster cells in vitro . Chromosoma 40, 183–192 (1972). https://doi.org/10.1007/BF00321463
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DOI: https://doi.org/10.1007/BF00321463