Summary
Electron microscopic observations have been made of the two epithelial cell types, light barrel-shaped and dark rod-shaped cells in the gall bladder of the mouse.
The light cells have a voluminous cytoplasm of low electron opacity in which cell organelles such as mitochondria, elements of granular endoplasmic reticulum, and free ribosomes undergo more or less degenerative changes. However, there are a relatively abundant Golgi apparatus and numerous lysosomal dense bodies. The ultrastructural features of the light cells suggest that they are an aged, degenerative cell type with declining functional activity and a high degree of hydration.
The dark cells are characterized by a high concentration of mitochondria and free ribosomes, more or less distinctive elements of granular endoplasmic reticulum, and well developed components of the Golgi apparatus. Such ultrastructural characteristics indicate that the dark cell type has a high synthetic activity.
What has been observed in the present study can well be correlated with the results of previous studies on the same cells by methods of light microscopic histochemistry.
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Bader, G.: Die submikroskopische Struktur des Gallenblasenepithels und seine Regeneration. 1. Mitt.: Karpfen (Cyprinus carpio, L.) und Frosch (Rana esculenta, L.). Z. mikr.-anat. Forsch. 74, 92–107 (1965a).
—: Die submikroskopische Struktur des Gallenblasenepithels. III. Mitt.: Das Epithel der Steingallenblase des Menschen. Frankfurt. Z. Path. 74, 502–511 (1965b).
Chapman, G. B., A. J. Chiarodo, R. J. Coffey, and K. Wieneke: The fine structure of mucosal epithelial cells of a pathological human gall bladder. Anat. Rec. 154, 579–616 (1966).
Duve, C. de: The lysosomes. In: The living cell. Readings from Scientific Americans, p. 72–80. San Francisco and London: W. H. Freeman & Co. (1965).
Evett, R. D., J. A. Higgins, and A. L. Brown: The fine structure of normal mucosa in human gall bladder. Gastroenterology 47, 49–60 (1964).
Ferner, H.: Über das Epithel der menschlichen Gallenblase. Z. Zellforsch. 34, 503–513 (1949).
Gompper, H.: Über das schleimartige Sekret der Gallenblase. Z. mikr.-anat. Forsch. 57, 280–303 (1951).
Hayward, A. F.: Aspects of the fine structure of the gall bladder epithelium of the mouse. J. Anat. (Lond.) 96, 227–236 (1962a).
—: Electron microscopic observations on absorption in the epithelium of the guinea pig gall bladder. Z. Zellforsch. 56, 197–202 (1962b).
—: The fine structure of the gall bladder epithelium of the sheep. Ibid. 65, 331–339 (1965).
—: An electron microscopic study of developing gall bladder epithelium in the rabbit. J. Anat. (Lond.) 100, 245–259 (1966).
Johnson, F. R., R. M. McMinn, and R. F. Birchenough: The ultrastructure of the gall bladder epithelium of the dog. Ibid. 96, 477–487 (1962).
Karnovsky, M. J.: Simple methods for “staining with lead” at high pH in electron microscopy. J. biophys. biochem. Cytol. 11, 729–732 (1961).
Kaye, G. I., H. O. Wheeler, R. T. Whitlock, and N. Lane: Fluid transport in the rabbit gall bladder. A combined physiological and electron microscopic study. J. Cell Biol. 30, 237–268 (1966).
Luft, J. H.: Improvements in Epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Miller, F., and G. E. Palade: Lytic activities in renal protein absorption droplets. An electron microscopical cytochemical study. J. Cell Biol. 23, 519–552 (1964).
Millonig, G.: A modified procedure for lead staining of thin sections. J. biophys. biochem. Cytol. 11, 736–739 (1961).
Mori, Sh.: Histology and histogenesis of the gall bladder in the mouse. Nagoya Igakkai Zasshi 47, 585–606 (1938).
Nagahiro, K.: Zytologische Untersuchungen über die Epithelzellen der Gallenblase des Menschen. Cytologia (Tokyo) 9, 132–163 (1938).
Novikoff, A. B.: Lysosomes and related particles. In: The Cell (J. Brachet, and A. E. Mirsky, eds.), vol. 2, p. 423–488. New York: Academic Press 1961.
—, E. Essner, S. Goldfischer, and M. Heus: Nucleoside phosphatase activities of cytomembranes. In: Symp. Internat. Society for Cell Biology, vol. 1, The interpretation of ultrastructure (Harris, R. J. C., ed.) p. 149–192. New York: Academic Press 1962.
Ott, H.: Das Gallengangsystem des Schweines (Sus scrofa domesticus). Z. Anat. Entwickl.-Gesch. 107, 7–17 (1937).
Pfuhl, W.: Die Gallenblase und die extrahepatischen Gallengänge. In: Handbuch der mikroskopischen Anatomie des Menschen, hrsgg. von W. v. Möllendorff, Bd. 5, S. 426–462. Berlin: Springer 1932.
Porter, K. R.: The ground substance; observations from electron microscopy. In: The Cell (J. Brachet, and A. E. Mirsky, eds.), vol. 2, p. 621–675. New York: Academic Press 1961.
Sabatini, D. D., K. Bensch, and R. J. Barnett: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzyme activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963).
Seeliger, M.: Über den Bau des Gallengangsystems bei Carnivoren (Hund und Katze) mit besonderer Berücksichtigung der Schleimbildung und des Glykogengehaltes. Z. Zellforsch. 26, 578–602 (1937).
Wallraff, J., u. K. F. Dietrich: Zur Morphologie und Histochemie der Steingallenblase des Menschen. Ibid. 46, 155–231 (1957).
Yamada, E.: The fine structure of the gall bladder epithelium of the mouse. J. biophys. biochem. Cytol. 1, 445–458 (1955).
Yamada, K.: Chemocytological observations on two peculiar epithelial cell types in the gall bladders of laboratory rodents. Z. Zellforsch. 56, 180–187 (1962).
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Yamada, K. Some observations on the fine structure of light and dark cells in the gall bladder epithelium of the mouse. Zeitschrift für Zellforschung 84, 463–472 (1967). https://doi.org/10.1007/BF00320862
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DOI: https://doi.org/10.1007/BF00320862