Abstract
The dependence of nucleolar structure on DNA and RNA synthesis in synchronous cultures of the slime mold Physarum polycephalum was traced through the mitotic cycle. The blockage of RNA synthesis produces a characteristic abnormality of the nucleolar structure when imposed at any time during interphase. But differences in the function of the early and late replicating DNA molecules were observed. The blockage of DNA synthesis causes abnormality of nucleolar structure only when imposed during the early part of the S-period.
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Birnstiel, M. L., Wallace, H., Sirlin, J. L., Fischberg, M.: Localisation of the ribosomal DNA complements in the nucleolar organizer region of Xenopus laevis. Nat. Cancer Inst. Monogr. 23, 431–448 (1966).
Braun, R., Evans, T. E.: Replication of nuclear satellite and mitochondrial DNA in the mitotic cycle of Physarum. Biochim. biophys. Acta (Amst.) 182, 511–522 (1969).
Braun, R., Mittermayer, C., Rusch, H. P.: Sequential temporal replication of DNA in Physarum polycephalum. Proc. nat. Acad. Sci. (Wash.) 53, 924–931 (1965).
Brown, D. D.: The nucleolus and synthesis of ribosomal RNA during oogenesis and embryogenesis of Xenopus laevis. Nat. Cancer Inst. Monogr. 23, 297–310 (1966).
Brown, D. D., Gurdon, J. B.: Absence of ribosomal RNA synthesis in the anucleolate mutant of Xenopus laevis. Proc. nat. Acad. Sci. (Wash.) 51, 139–146 (1964).
Busch, H., Smetana, K.: Effects of drugs and other agents on the nucleolus. In: The nucleolus (H. Busch and K. Smetana, eds.), p. 473–510. New York-London: Acad. Press 1970.
Daniel, J. W., Baldwin, H. H.: Methods of culture for plasmodial myxomycetes. In: Methods in cell physiology (D. M. Prescott, ed.), vol.I, p. 9. New York: Acad. Press 1964.
Desjardins, R., Grogan, D. E., Arendell, J. P., Busch, H.: Effects of antitumor agents on the synthesis of nucleolar DNA. Cancer Res. 17, 159–164 (1967).
German, J. L.: The pattern of DNA synthesis in the chromosomes of human blood cells. J. Cell Biol. 20, 37–55 (1964).
Ghosh, S., Lettré, R., Ghosh, I.: DNA synthetic patterns in the nucleolus II. Chicken fibroblasts. J. Cell Biol. 45, 177–181 (1970).
Gilbert, C. W., Muldal, S., Lajtha, L. G., Rowley, J.: Time-sequence of human chromosome duplication. Nature (Lond.) 195, 869 (1962).
Greenberg, L. J., Uhr, J. W.: Myeloma tumor satellite DNA: A role in ribosomal RNA synthesis. Biochem. biophys. Res. Commun. 27, 523–528 (1967).
Guttes, S., Guttes, E., Ellis, R. A.: Electron microscope study of mitosis in Physarum polycephalum. J. Ultrastruct. Res. 22, 508–529 (1968).
Harris, H.: The initiation of DNA synthesis in the connective tissue cell, with some observations on the function of the nucleolus. Biochem. J. 72, 54–60 (1959).
Holt, C. E., Gurrey, E. G.: Minor components of the DNA of Physarum polycephalum. Cellular location and metabolism. J. Cell Biol. 40, 484–496 (1969).
Journey, L. J., Goldstein, M. N.: Electron microscope studies on HeLa cell lines sensitive and resistant to actinomycin D. Cancer Res. 21, 929–932 (1961).
Kajiwara, K., Mueller, G. C.: Molecular events in the reproduction of animal cells. III. Fractional synthesis of deoxyuridine and its effects on cloning efficiency. Biochim. biophys. Acta (Amst.) 91, 486–493 (1964).
Kasten, F. H., Strasser, F. F.: Nucleic acid synthetic patterns in synchronized mammalian cells. Nature (Lond.) 211, 135–140 (1966).
Kume, F., Maruyama, S., D'Agostino, A. N., Chiga, M.: Nucleolar change produced by mithramycin in rat hepatic cell. Exp. molec. Path. 6, 254–260 (1967).
Lapis, K., Benedeczky, J.: Antimetabolic-induced changes in the fine structure of tumour cells. Acta biol. Acad. Sci. hung. 17, 199–215 (1966).
Lima-de-Faria, A.: DNA replication and gene amplification in the heterochromatin. In: Handbook of molecular cytology (A. Lima-de-Faria, ed.), p. 277–325. Amsterdam-London: North-Holland Publ. Comp. 1969.
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol 9, 409 (1961).
Matsuda, K., Siegel, A.: Hybridization of plant ribosomal RNA to DNA: The isolation of a DNA component rich in ribosomal RNA cistrons. Proc. nat. Acad. Sci. (Wash.) 58, 673–680 (1967).
Miller, O. L., Jr., Beatty, B. R.: Nucleolar structure and function. In: Handbook of molecular cytology (A. Lima-de-Faria, ed.), p. 605–619. Amsterdam, London: North-Holland Publ. Comp. 1969.
Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Physiol. 32, 1637 (1961).
Mittermayer, C., Braun, R., Rusch, H. P.: RNA synthesis in the mitotic cycle of Physarum polycephalum. Biochim. biophys. Acta (Amst.) 91, 399–405 (1964).
Muramatsu, M., Hodnett, L., Steele, W. J., Busch, H.: Molecular properties of RNA of isolated nucleoli of rat liver. I. Synthesis of 28 S RNA in the nucleolus. Biochim. biophys. Acta (Amst.) 123, 116–125 (1966).
Narayan, K. S., Steele, W. J., Busch, H.: Evidence that the granular and fibrillar elements of nucleoli contain 28 S and 6 S RNA, respectively. Exp. Cell Res. 43, 483–492 (1966).
Nygaard, O., Guttes, E., Rusch, H. P.: Nucleic acid metabolism in slime mold with synchronous mitosis. Biochim. biophys. Acta (Amst.) 38, 298–306 (1960).
Panteleakis, P. M., Mateyko, G. M.: Effects of actinomycin D on the nucleoli of different cells in vitro. J. Cell Biol. 31, 154A (1966).
Penman, S.: RNA metabolism in the HeLa nucleus. J. molec. Biol. 17, 117–130 (1966).
Perry, R. P.: Nucleoli: the cellular sites of ribosome production. In: Handbook of molecular cytology (A. Lima-de-Faria, ed.), p. 621–636. Amsterdam-London: North-Holland publi. Comp. 1969.
Perry, R. P., Hell, A., Errera, M.: The role of the nucleolus in ribonucleic acid and protein synthesis. I. Incorporation of cytidine into normal and nucleolar inactivated HeLa cells. Biochim. biophys. Acta (Amst.) 49, 47–57 (1961).
Quagliarotti, G., Hidvegi, E., Wikman, J., Busch, H.: Structural analysis of nucleolar precursors of ribosomal RNA. II. Comparative hybridization of nucleolar and ribosomal RNA with nucleolar DNA. J. biol. Chem. 245 (1970).
Rao, B., Gontcharoff, M.: Functionality of newly synthesized DNA as related to RNA synthesis during mitotic cycle in Physarum polycephalum. Exp. Cell Res. 56, 269–274 (1969).
Ribas-Mundo, M.: Nucleolar DNA synthesis in leukocytes cultured in vitro. Exp. Cell Res. 41, 210–214 (1966).
Simard, R., Bernhard, W. A.: Le phénomène de la segrégation nucléolaire: Spécificité d'action de certains antimétabolites. Intern. J. Cancer 1, 463–479 (1966).
Stenram, U.: The effects of fluorouracil and actinomycin, single and combined on the nucleolar ultrastructure of various tissues of the rat. Z. Zellforsch. 94, 282–292 (1969).
Stubblefield, E., Mueller, G. C.: Molecular events in the reproduction of animal cells. II. The focalized synthesis of DNA in the chromosomes of HeLa cells. Cancer Res. 22, 1091 (1962).
Taylor, J. H.: Asynchronous duplication of chromosomes in cultured cells of Chinese hamster. J. biochem. biophys. Cytol. 7, 455 (1960).
Zellweger, A., Braun, A.: RNA of Physarum. II. Template transcription in the mitotic cycle. Exp. Cell Res. 65, 424–432 (1971).
Zellweger, A., Braun, R.: RNA of Physarum. I. Preparation and properties. Exp. Cell Res. 65, 413–423 (1971).
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Gontcharoff, M., Rao, B. Dependence of the nucleolar structure on DNA and RNA synthesis. Chromosoma 38, 441–457 (1972). https://doi.org/10.1007/BF00320162
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DOI: https://doi.org/10.1007/BF00320162