Skip to main content
SpringerLink
Log in

Alteration of ringer pH and its effects on precursor incorporation into Drosophila salivary gland nuclei

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

Drosophila salivary glands were explanted and incubated with 3H-uridine (or 3H-thymidine) in Ringer's solution (Ephrussi-Beadle modified saline) adjusted to pH values in the integral range, 4 to 10. The results of autoradiographic investigations indicate a differential effect of altering Ringer pH on 3H-uridine as opposed to 3H-thymidine incorporation: a) Relatively uniform levels of chromosomal incorporation of 3H-thymidine occurred over the range of test pH. Some decrease of incorporation was noted at pH 5 and some increase at pH 9. b) Chromosomal incorporation of 3H-uridine was severely depressed at pH 4 and 7 relative to the high incorporation levels observed at other pH values. Controlling pH of Ephrussi-Beadle Ringer's solution in such experiments seems a necessity. This appears especially important for studies involving 3H-uridine incorporation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Caldwell, P. C.: Intracellular pH. Int. Rev. Cytol. 5, 229–277 (1956)

    Google Scholar 

  • Ceccarini, C., Eagle, H.: pH as a determinant of cellular growth and contact inhibition. Proc. nat. Acad. Sci. (Wash.) 68, 229–233 (1971)

    Google Scholar 

  • Ephrussi, B., Beadle, G. W.: A technique of transplantation for Drosophila. Amer. Naturalist 70, 218–225 (1936)

    Google Scholar 

  • Gruzdev, A. D., Belaja, A. N.: The influence of pH, tonicity and ionic strength of solutions on the size of polytene chromosomes. (In Russian). Tsitologia (U.S.S.R.) 10, 297–305 (1968)

    Google Scholar 

  • Lacko, L., Wittke, B., Geck, P.: The pH dependence of exchange transport in human erythrocytes. J. cell. Physiol. 80, 73–78 (1971)

    Google Scholar 

  • Leake, R. E., Trench, M. E., Barry, J. M.: Chromosomes isolated from unfixed salivary glands of Chironomus. In: Developmental studies on giant chromosomes (W. Beermann, ed.), p. 35–57, Berlin-Heidelberg-New York: Springer 1972

    Google Scholar 

  • Maroni, G., Plaut, W.: Dosage compensation in Drosophila melanogaster triploids. I. Autoradiographic study. Chromosoma (Berl.) 40, 361–377 (1973)

    Google Scholar 

  • Rubin, H.: pH and population density in the regulation of animal cell multiplication. J. Cell Biol. 51, 686–702 (1971)

    Google Scholar 

  • Sorokina, Z. A.: Role of hydrogen ions in active potassium and sodium transport across the neuronal membrane of Helix pomatia. Translated from: Zh. Evol. Biokhim. i Fiziol. 8, 35–40 (1972)

    Google Scholar 

  • Waddell, W. J., Bates, R. G.: Intracellular pH. Physiol. Rev. 49, 285–329 (1969)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Zoology, University of Wisconsin, 53706, Madison, Wisconsin, USA

    Jean G. Heitz & Walter Plaut

Authors
  1. Jean G. Heitz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Walter Plaut
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heitz, J.G., Plaut, W. Alteration of ringer pH and its effects on precursor incorporation into Drosophila salivary gland nuclei. Chromosoma 51, 147–155 (1975). https://doi.org/10.1007/BF00319832

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00319832

Keywords

Search

Navigation