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Enhanced extrinsic innervation of nasal and oral chemosensory mucosae in keratin 14-NGF transgenic mice

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Abstract

The role of nerve growth factor (NGF) in neurotrophic support for the extrinsic innervation of the nasal and oral mucosae was investigated in keratin 14 (K14)-NGF transgenic mice in which NGF was over-expressed in K14-synthesizing cells. K14 immunoreactivity was localized in the epithelial basal cells of the whisker pad skin, the hard palate, the floor of the ventral meatus, and the anterior tongue that are stratified squamous epithelia, and also in basal cells of the vomeronasal, olfactory, and respiratory epithelia that are non-stratified epithelia. In transgenic mice, NGF expression was identified and confined primarily to the basal cells of stratified epithelia. The nasal mucosae including the vomeronasal, olfactory, and respiratory mucosae, and the glands associated with the vomeronasal organ received a greater innervation of protein gene product 9.5-immunoreactive extrinsic fibers in transgenic animals than nontransgenic controls. An increased density of calcitonin gene-related peptide-immunoreactive extrinsic fibers was observed in the nonsensory epithelia of the vomeronasal organ, the olfactory sensory and respiratory epithelia in transgenic animals. Our results indicated that the hyperinnervation of the nasal and oral mucosae by extrinsic neurons is due at least partially to target-derived NGF synthesis and release by K14-expressing basal cells.

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Authors and Affiliations

  1. Department of Physiology, University of Kentucky College of Medicine, Lexington, Kentucky, USA

    Shigeru Takami & Thomas V. Getchell

  2. Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Kentucky College of Medicine, Lexington, Kentucky, USA

    Marilyn L. Getchell, Masuo Yamagishi & Thomas V. Getchell

  3. Sanders-Brown Center on Aging, University of Kentucky College of Medicine, Lexington, Kentucky, USA

    Marilyn L. Getchell & Thomas V. Getchell

  4. Department of Pathology, University of Kentucky College of Medicine, Lexington, Kentucky, USA

    Kathryn M. Albers

Authors
  1. Shigeru Takami
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  2. Marilyn L. Getchell
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  3. Masuo Yamagishi
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  4. Kathryn M. Albers
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  5. Thomas V. Getchell
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Additional information

This work was supported by NIH grants NIDCD-00159 (T.V.G.), NIDCO-01715 (M.L.G.), and NINDS-31826 (K.M.A.).

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Takami, S., Getchell, M.L., Yamagishi, M. et al. Enhanced extrinsic innervation of nasal and oral chemosensory mucosae in keratin 14-NGF transgenic mice. Cell Tissue Res 282, 481–491 (1995). https://doi.org/10.1007/BF00318880

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  • DOI: https://doi.org/10.1007/BF00318880

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