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Occurrence, distribution and possible role of the regulatory peptide endothelin in the nasal mucosa

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Abstract

Nasal blood flow is finely regulated by local release of neurotransmitters, neuropeptides and other bioactive molecules acting via paracrine mechanisms. We have investigated the occurrence and distribution in human nasal mucosa of endothelin, a potent vasoconstrictor peptide, by immunocytochemistry and the effect of systemic administration of endothelin-1 on vascular perfusion of rabbit nasal mucosa by laser Doppler flowmetry. Endothelin-like immunoreactivity was demonstrated within vascular endothelial cells in both developing and mature human mucosa. Nasal epithelial cells and some connective tissue cells, presumed to be macrophages, also displayed specific immunostaining. In rabbits injected with endothelin-1, a potent and prolonged nasal vasoconstriction was observed. It is suggested that endothelin released locally may participate in the regulation of nasal blood flow via paracrine mechanisms. Since endothelin has growth-promoting actions on several cell types, it is also tentatively proposed that this regulatory peptide may play a role during development of the nose.

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References

  • Baley PA, Resink TJ, Eppenberger U, Hahn AWA (1990) Endothelin messenger RNA and receptors are differentially expressed in cultured human breast epithelial and stromal cells. J Clin Invest 85:1320–1323

    Google Scholar 

  • Baraniuk JN, Lundgren JD, Okayama M, Mullol J, Merida M, Shelhamer JH, Kaliner MA (1990a) Vasoactive intestinal peptide in human nasal mucosa. J Clin Invest 86:825–831

    Google Scholar 

  • Baraniuk JN, Castellino S, Lundgren JD, Goff J, Mullol J, Merida M, Shelhamer JH, Kaliner MA (1990b) Neuropeptide Y (NPY) in human nasal mucosa. Am J Respir Cell Mol Biol 3:165–173

    Google Scholar 

  • Barnes PJ (1989) Regulatory peptides in the respiratory system. In: Polak JM (ed) Regulatory Peptides. Birkhäser, Basel, pp 317–333

    Google Scholar 

  • Black PN, Ghatei MA, Takahashi K, Bretherton-Watt D, Krausz T, Dollery CT, Bloom SR (1989) Formation of endothelin by cultured airway epithelial cells. FEBS Lett 255:129–132

    Google Scholar 

  • Casasco A, Calligaro A, Casasco M, Springall DR, Tenti P, Marchetti C, Poggi P, Polak JM (1991) Immunohistochemical localization of endothelin-like immunoreactivity in human tooth germ and mature dental pulp. Anat Embryol (Berl) 183:515–520

    Google Scholar 

  • Casey LM, Word RA, MacDonald PC (1991) Endothelin-1 gene expression and regulation of endothelin mRNA and protein biosynthesis in avascular human amnion. J Biol Chem 266:5762–5768

    Google Scholar 

  • Ehrenreich H, Anderson RW, Fox CH, Rieckmann P, Hoffman GS, Travis WD, Coligan JE, Kehrl JH, Fauci AS (1990) Endothelins, peptides with potent vasoactive properties are produced by human macrophages. J Exp Med 172:1741–1748

    Google Scholar 

  • Giaid A, Gibson SJ, Ibrahim NBN, Legon S, Bloom SR, Yanagisawa M, Masaki T, Varndell IM, Polak JM (1989) Endothelin 1, an endothelium-derived peptide, is expressed in neurons of the human spinal cord and dorsal root ganglia. Proc Natl Acad Sci USA 86:7634–7638

    Google Scholar 

  • Giaid A, Polak JM, Gaitonde V, Hamid QA, Moscoso G, Legon S, Uwanogho D, Roncalli M, Shinmi O, Sawamura T, Kimura S, Yanagisawa M, Masaki T, Springall DR, (1991a) The distribution of endothelin-like immunoreactivity and mRNA in the developing and adult human lung. Am J Resp Cell Mol Biol 4:50–58

    Google Scholar 

  • Giaid A, Gibson SJ, Herrero MT, Gentleman S, Legon S, Yanagisawa M, Masaki T, Ibrahim NBN, Roberts GW, Rossi ML, Polak JM (1991b) Topographical localisation of endothelin mRNA and peptide immunoreactivity in neurones of the human brain. Histochemistry 95:303–314

    Google Scholar 

  • Hemsen A, Lundberg JM (1991) Presence of endothelin-1 and endothelin-3 in peripheral tissues and central nervous system of the pig. Regul Pept 36:71–83

    Google Scholar 

  • Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577–580

    Google Scholar 

  • Inoue A, Yanagisawa M, Kimura S, Kasuya Y, Miyauchi T, Goto K, Masaki T (1989) The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes. Proc Natl Acad Sci USA 86:2863–2867

    Google Scholar 

  • Komuro I, Kurihara H, Sugiyama T, Takaku F, Yazaki Y (1988) Endothelin stimulates c-fos and c-myc expression and proliferation of vascular smooth muscle cells. FEBS Lett 238:249–252

    Google Scholar 

  • Loesch A, Bodin P, Burnstock G (1991) Colocalization of endothelin, vasopressin and serotonin in cultured endothelial cells of rabbit aorta. Peptides 12:1095–1103

    Google Scholar 

  • MacCumber MW, Ross CA, Glaser BM, Snyder SH (1989) Endothelin: visualization of mRNAs by in situ hybridization provides evidence for local action. Proc Natl Acad Sci USA 86:7285–7289

    Google Scholar 

  • Miyauchi T, Tomobe Y, Shiba R, Ishikawa T, Yanagisawa M, Kimura S, Sugishita Y, Iwao I, Goto K, Masaki T (1990) Involvement of endothelin in the regulation of human vascular tonus potent vasoconstrictor effect and existence in endothelial cells. Circulation 81:1874–1880

    Google Scholar 

  • Orlando C, Brandi ML, Peri A, Giannini S, Fantoni G, Calabresi E, Serio M, Maggi M (1990) Neurohypophyseal hormone regulation of endothelin secretion from rabbit endomethial cells in primary culture. Endocrinology 126:1780–1782

    Google Scholar 

  • Polak JM, Van Noorden S (1987) Specificity problems and essential controls. In: Hammond C (ed) An introduction to immunocytochemistry: current techniques and problems. Oxford University Press, Microscopical Society, Oxford, pp 33–36

    Google Scholar 

  • Power RF, Wharton J, Zhao Y, Bloom SR, Polak JM (1989) Autoradiographic localization of endothelin-1 binding sites in the cardiovascular and respiratory systems. J Cardiovasc Pharmacol 13 [Suppl 5]:S50-S56

    Google Scholar 

  • Rozengurt N, Springall DR, Polak JM (1990) Localization of endothelin-like immunoreactivity in airway epithelium of rat and mice. J Pathol 160:5–8

    Google Scholar 

  • Shinmi O, Kimura S, Yoshizawa T, Sawamura T, Uchiyama Y, Sugita Y, Kanazawa I, Yanagisawa M, Goto K, Masaki T (1989) Presence of endothelin-1 in porcine spinal cord: isolation and sequence determination. Biochem Biophys Res Commun 162:340–346

    Google Scholar 

  • Springall DR, Howarth PW, Counihan H, Djukanovic R, Holgate ST, Polak J (1991) Endothelin immunoreactivity of the airway epithelium is elevated in asthmatic patients. Lancet 337:697–701

    Google Scholar 

  • Stefanini M, de Martino C, Zamboni L (1967) Fixation of ejaculated spermatozoa for electron microscopy. Nature 216:173–174

    Google Scholar 

  • Stjarne P, Lacroix JS, Anggard A, Lundberg MJ (1991) Release of calcitonin gene-related peptide in the pig nasal mucosa by antidromic nerve stimulation and capsaicin. Regul Pept 33:251–262

    Google Scholar 

  • Takuwa N, Takuwa Y, Yanagisawa M, Yamashita K, Masaki T, (1989) A novel vasoactive peptide endothelin stimulates mitogenesis through inositol lipid turnover in swiss 3T3 fibroblasts. J Biol Chem 264:7856–7861

    Google Scholar 

  • Uchida Y, Ninomiya H, Saotome M, Nomura A, Ohtsuka M, Yanagisawa M, Goto K, Masaki T, Hagesawa S (1988) Endothelin a novel vasonstrictor peptide, as potent broncocostrictor. Eur J Pharmacol 154:227–228

    Google Scholar 

  • Vane JR, Anggard EE, Botting RM (1990) Regulatory functions of the vascular endothelium. N Engl J Med 323:27–36

    Google Scholar 

  • Wiklund NP, Ohlen A, Cederqvist B (1988) Inhibition of adrenergic neuroeffector transmission by endothelin in the guinea-pig femoral artery. Acta Physiol Scand 134:311–312

    Google Scholar 

  • Wiklund NP, Ohlen A, Wiklund CU, Hedqvist P, Gustafsson LE (1990) Endothelin modulation of neuroeffector transmission in rat and guinea pig vas deferens. Eur J Pharmacol 185:25–33

    Google Scholar 

  • Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K, Masaki T (1988a) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332:411–415

    Google Scholar 

  • Yanagisawa M, Inoue A, Ishikawa T, Kasuya Y, Kimura S, Kumagaye S-I, Nakajima K, Watanabe TX, Sakakibara S, Goto K, Masaki T (1988b) Primary structure, synthesis and biological activity of rat endothelin, an endothelin-derived vasoconstrictor peptide. Proc Natl Acad Sci USA 85:6964–6967

    Google Scholar 

  • Yoshizawa T, Kimura S, Kanazawa I, Uchiyama Y, Yanagisawa M, Masaki T (1989) Endothelin localizes in dorsal horn and acts on the spinal neurones: possible involvement of dihydropyridine-sensitive calcium channels and substance P release. Neurosci Lett 102:179–184

    Google Scholar 

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Authors and Affiliations

  1. Institute of Histology and Embryology, University of Pavia, Via Forlanini, 10, I-27100, Pavia, Italy

    Andrea Casasco, Marco Casasco, Antonia Icaro Cornaglia & Alberto Calligaro

  2. Department of Otorhynolaringology, I.R.C.C.S. “S.Matteo” Hospital, I-27100, Pavia, Italy

    Marco Benazzo & Eugenio Mira

  3. Histochemistry Unit, Royal Postgraduate Medical School, Hammersmith Hospital, W12 ONN, London, UK

    David R. Springall & Julia M. Polak

Authors
  1. Andrea Casasco
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  2. Marco Benazzo
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  3. Marco Casasco
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  4. Antonia Icaro Cornaglia
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  5. David R. Springall
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  6. Alberto Calligaro
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  7. Eugenio Mira
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  8. Julia M. Polak
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Casasco, A., Benazzo, M., Casasco, M. et al. Occurrence, distribution and possible role of the regulatory peptide endothelin in the nasal mucosa. Cell Tissue Res 274, 241–247 (1993). https://doi.org/10.1007/BF00318743

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  • DOI: https://doi.org/10.1007/BF00318743

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