Abstract
Male NMRI mice were exposed to benzene in air, concentrations ranging between 1–200 ppm. The following parameters in the bone marrows were examined:
1. number of nucleated cells/tibia, 2. number of colony forming granulopoietic stem cells (CFU-C)/tibia, and 3. frequency of micronuclei in polychromatic erythrocytes.
At continuous exposure as low benzene concentrations as 21 ppm during 4–10 days significantly affected the three parameters. Intermittent exposure (8 h/day, 5 days/week, 2 weeks) also resulted in measurable toxicity, particularly on number of CFU-C/tibia and frequency of micronuclei, at 21 ppm and higher doses. Short peak exposures had very limited effects but did increase the proliferation rate of the bone marrow, i.e., the number of CFU-C/105 cells became elevated.
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References
Gill DP, Kempen RR, Nash JB, Ellis S (1979) Modifications of benzene myelotoxicity and metabolism by phenobarbital, SKF-525 A and 3-methylcholanthrene. Life Sci 25: 1633–1640
Goldstein BD (1977) Hematotoxicity in humans. J Toxicol Environ Health Suppl 2: 69–106
Green JD, Snyder CA, LoBue J, Goldstein BD, Albert RE (1981a) Acute and chronic dose/response effects of inhaled benzene on multipotential hematopoietic stem (CFU-S) and granulocyte/macrophage progenitor (GM-CFU-C) cells in CD-1 mice. Toixicol Appl Pharmacol 58: 492–503
Green JD, Snyder CA, LoBue J, Goldstein BD, Albert RE (1981b) Acute and chronic dose/response effect of benzene inhalation on the peripheral blood, bone marrow and spleen cells of CD-1 mice. Toxicol Appl Pharmacol 59: 204–214
Harigaya K, Miller ME, Cronkite EP, Drew RT (1981) The detection of in vivo hematotoxicity of benzene by in vitro liquid bone marrow cultures. Toxicol Appl Pharmacol 60: 346–353
Hite M, Pecharo M, Smith I, Thornton S (1980) The effect of benzene in the micronucleus test. Mutat Res 77: 149–155
Irons RD, Neptun DA (1980) Effects of the principal hydroxymetabolites of benzene on microtubule polymerization. Arch Toxicol 45: 297–305
Laskin S, Goldstein BD (1977) Benzene toxicity: a critical evaluation. J Toxicol Environ Health Suppl 2
Lee EW, Kocsis JJ, Snyder R (1973) Dose dependent inhibition of 59Fe incorporation into erythrocytes after a single dose of benzene. Res Commun Chem Pathol Pharmacol 5: 547–550
Meyne J, Legator MS (1980) Sex-related differences in cytogenetic effects of benzene in the bone marrow of Swiss mice. Environ Mutat 2: 43–50
NIOSH, U.S. Department of health, education and welfare (1974) Criteria for a recommended standard. Occupational exposure to benzene. HEW publication no. (NIOSH) 74–137
Santesson CG (1897) Über chronische Vergiftungen mit Steinkohlentheerbenzin; vier Todesfälle. Arch Hyg Berl 31: 336–376
Schmid W (1975) The micronucleus test. Mutat Res 31: 9–15
Siou MG, Conan L (1977) Activité mutagène du benzène et du benzo(a)pyrène: Mise en évidence par la technique des corps de Howell-Jolly (micronucleus test). Cah Notes Doc 69: 433–444
Snyder R, Kocsis JJ (1975) Current concepts of chronic benzene toxicity. CRC Crit Rev Toxicol 3: 265–288
Tice RR, Costa DL, Drew RT (1980) Cytogenetic effects of inhaled benzene in murine bone marrow: Induction of sister chromatid exchanges, chromosomal aberrations and cellular proliferation inhibition in DBA/2 mice. Proc Natl Acad Sci USA 77: 2148–2152
Tunek A (1981) Metabolism and toxicity of benzene. Academic dissertation. University of Lund, Sweden
Tunek A, Olofsson T, Berlin M (1981) Toxic effects of benzene and benzene metabolites on granulopoietic stem cells and bone marrow cellularity in mice. Toxicol Appl Pharmacol 59: 149–156
Tunek A, Högsted B, Olofsson T (1982) Mechanism of benzene toxicity. Effects of benzene and benzene metabolites on bone marrow cellularity, number of granulopoietic stem cells and frequency of micronuclei in mice. Chem-Biol Interact 39: 129–138
Uyeki EM, El Ash-kar S, Shoeman DW, Bisel TU (1977) Acute toxicity of benzene inhalation to hemopoietic precursor cells. Toxicol Appl Pharmacol 40: 49–57
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Toft, K., Olofsson, T., Tunek, A. et al. Toxic effects on mouse bone marrow caused by inhalation of benzene. Arch Toxicol 51, 295–302 (1982). https://doi.org/10.1007/BF00317008
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DOI: https://doi.org/10.1007/BF00317008