Summary
Immunocytochemical reaction for demonstration of calbindin D-28K has been performed in superficial pineal bodies of the Mongolian gerbil (Meriones unguiculatus) and the rat. Whereas in the Mongolian gerbil there were no clearly expressed calbindin immunoreactive cells, these were numerous in the rat pineal body. Here the calbindin-positive cells — probably pinealocytes — were disposed along capillaries. In view of the role of calbindin in binding and transporting calcium and regulating its intracellular levels, the absence of this protein in the gerbil pineal body has been interpreted as signifying the inability of pinealocytes to eliminate intracellular calcium with possible consequent formation of acervuli.
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References
Baimbridge KG, Miller (1982) Immunohistochemical localization of calcium-binding protein in the cerebellum, hippocampal formation and olfactory bulb of the rat. Brain Res 245:223–229
Balmain N, Brehier A, Cuisinier-Gleizes P, Mathieu H (1986) Evidence for the presence of Calbindin-D 28K (CaBP-28K) in the tibial growth cartilages of rats. Cell Tissue Res 245:331–335
Bargmann W (1943) Die Epiphysis cerebri. In: Möllendorff W (ed) Handbuch der mikroskopischen Anatomie des Menschen, Vol VI/4. Springer, Berlin, pp 434–445
Celio MR, Norman AW (1985) Nucleus basalis Meynert neurons contain the vitamin D-induced calcium-binding protein (Calbindin-D 28K). Anat Embryol 173:143–148
Fullmer CS, Wasserman RH (1975) Isolation and partial characterization of intestinal calcium-binding proteins from the cow pig, borse, guinea pig, and chick. Biochem Biophys Acta 393:134–142
Japha JL, Eder TJ, Goldsmith ED (1974) Morphological and histochemical features of the gerbil pineal system. Anat Rec 178:381–382
Japha JL, Eder TJ, Goldsmith ED (1976) Calcified inclusions in the superficial pineal gland of the Mongolian gerbil, Meriones unguiculatus. Acta Anat 94:533–544
Garcia-Segura LM, Beatens D, Roth J, Norman AW, Orci L (1984) Immunohistochemical mapping of calcium-binding protein immunoreactivity in the rat central nervous system. Brain Res 296:75–86
Jande SS, Tolnai S, Lawson DEM (1981) Immunocytochemical localization of vitamin D-dependent calcium-binding protein in duodenum, kidney, uterus, and cerebellum of chickens. Histochemistry 71:99–116
Kowarski S, Schachter D (1980) Intestinal membrane calcium binding protein vitamin D-dependent membrane component of the intestinal calcium transport mechanism. J Biol Chem 255:10834–10840
Krstié R (1986) Pineal calcification: its mechanism and significance. J Neural Transm [Suppl] 21:415–432
Philippe E, Droz B (1986) Effect of skeletal muscle on the expression of a calcium-binding protein (Calbindin) in dorsal root ganglia of the chick embryo. Abstracts of the Society for Neuroscience. 16th Annual Meeting, Washington DC, Part 2, 1125
Philippe E, Droz B (1987) Calbindin D-28k-immunoreactive neurons in chick dorsal root ganglion: Ontogenesis and cytological characteristics of the immunoreactive sensory neurons. Neuroscience (in press)
Rabié A, Thomasset M, Legrand Ch (1983) Immunocytochemical detection of calcium-binding protein in the cochlear and vestibular hair cells of the rats. Cell Tissue Res 232:691–696
Rabié A, Thomasset M, Parkes CO, Clavel MC (1985) Immunocytochemical detection of 28000-MW calcium-binding protein in horizontal cells in the rat retina. Cell Tissue Res 240:493–496
Rhoten WB, Christakos S (1981) Immunocytochemical localization of vitamin D-dependent calcium-binding protein in mammalian nephron. Endocrinology 109:981–983
Roth J, Brown D, Norman AW, Orci L (1982) Localization of the vitamin D-dependent calcium-binding protein in mammalian kidney. Am J Phyisol 243:F243-F252
Schreiner DS, Jande SS, Parkes CO, Lawson DEM, Thomasset M (1983) Immunocytochemical demonstration of two vitamin D-dependent calcium-binding proteins in mammalian kidney. Acta Anat 117:1–14
Sternberger LA (1979) Immunocytochemistry. John Wiley & Sons, New York, pp 104–169
Taylor AN, Gleason WA, Lankford GL (1984) Immunocytochemical localization of rat intestinal vitamin D-dependent calciumbinding protein. J Histochem Cytochem 32:153–158
Vollrath L (1981) The pineal organ. In: Oksche A, Vollrath L (eds) Handbuch der mikroskopischen Anatomie des Menschen, Vol VI/7. Springer, Berlin Heidelberg New York, pp 225–230
Wasserman RH, Feher JJ (1978) Vitamin D-dependent calciumbinding proteins. In: Wasserman RH et al. (eds) Calcium binding proteins and calcium function. Proc Intern Symp on Calcium-binding proteins and calcium function in health and disease. Elsevier North Holland, New York, 292–302
Wasserman RH, Taylor AN (1966) Vitamin D3-induced calcium binding protein in chick intestinal mucosa. Science 152:791–793
Welsh MG (1984) Cytochemical analysis of calcium distribution in the superficial pineal gland of the Mongolian gerbil. J Pineal Res 1:305–316
Welsh MG, Reiter RJ (1978) The pineal gland of the gerbil, Meriones unguiculatus. Cell Tissue Res 193:323–336
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Dedicated to Prof. Dr. Johannes Lang on the occasion of his 5th birthday
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Krstié, R. Comparison of calbindin D-28K immunoreactivity in superficial pineal bodies of mongolian gerbil and rat. Anat Embryol 177, 349–352 (1988). https://doi.org/10.1007/BF00315843
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DOI: https://doi.org/10.1007/BF00315843