Skip to main content
SpringerLink
Log in

Peripheral avian yolk assemblage and its persistence in the blastoderm, studied by trypan blue-induced fluorescence

  • Published:
Anatomy and Embryology Aims and scope Submit manuscript

Summary

Shortly after subcutaneous or intraperitoneal injection of nontoxic quantities of trypan blue into laying Japanese quails, red fluorescent yolk granules appear in the peripheral ooplasm of their oocytes at the end of the lampbrush stage or subsequently. Later a red fluorescence can be observed in the apical cytoplasm of the granulosa cells. The results obtained by this method confirm our previous results (Callebaut 1974) obtained by autoradiography after 3H-leucine administration and furnish interesting additional data. The trypan blue-induced fluorescence method gives a good indication of the permeability of the oocytal cortex and its derivative the germinal disc. The avian yolk which is, or has been peripherally assembled (primordial, true white and yellow yolk) can be characteristically labelled by the administration of trypan blue. The injection of higher, still nontoxic quantities of trypan blue has a prolonged “retarding” effect and permits the marking of a broader part of the germinal disc or eventually of the blastoderm which develops from it.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson LM, Telfer WH (1970) Trypan blue inhibition of yolk deposition — a clue to follicle cell function in the Cecropia moth. J Embryol Exp Morphol 23:52–55

    Google Scholar 

  • Bacon WL, Koontz M (1971) Ovarian follicular growth and maturation in Coturnix quail. Poult Sci 50:233–236

    Google Scholar 

  • Bain JM, Hall JM (1969) Observations on the development and structure of the vitelline membrane of the hen's egg: an electron microscope study. Aust J Biol Sci 22:653–665

    Google Scholar 

  • Bartelmez GW (1912) The bilaterality of the pigeon's egg. I. A study in egg organization from the first growth period of the oocyte to the beginning of cleavage. J Morphol 23:269–328

    Google Scholar 

  • Beams HW, Kessel RG (1969) Synthesis and deposition of oocyte envelopes (vitelline membrane, chorion) and the uptake of yolk in the dragonfly. J Cell Sci 4:241–264

    Google Scholar 

  • Bellairs, R (1958) The conversion of yolk into cytoplasm in the chick blastoderm as shown by electron microscopy. J Embryol Exp Morphol 6:149–161

    Google Scholar 

  • Bellairs R, Harkness M, Harkness RD (1963) The vitelline membrane of the hen's egg: a chemical and electron microscopical study. J Ultrastruct Res 8:339–359

    Google Scholar 

  • Blanquet P, Stoll R, Maraud R, Mounier J, Meyniel G (1957) Sur la concentration du radioiode dans l'ovaire de la poule. C R Soc Biol 151:104–107

    Google Scholar 

  • Callebaut M (1968) Extracorporal development of quail oocytes. Experientia 24:1242–1243

    Google Scholar 

  • Callebaut M (1970) Extranuclear 3H-thymidine incorporation in subcortical cytoplasmic organelles of primary oocytes in the Japanese quail (Coturnix coturnix japonica). Experientia 26:1134

    Google Scholar 

  • Callebaut M (1971) Autoradiographic evidence for cytoplasmic DNA synthesis during the early final growth period in the oocytes of the Japanese quail (Coturnix coturnix japonica). Experientia 27:305–306

    Google Scholar 

  • Callebaut M (1972) Cytoplasmic uniaxial radial symmetry during the early final growth period in the oocytes of the Japanese quail. Experientia 28:62–63

    Google Scholar 

  • Callebaut M (1973) Correlation between germinal vesicle and oocyte development in the adult Japanese quail. A cytochemical and autoradiographic study. J Embryol Exp Morphol 29:145–157

    Google Scholar 

  • Callebaut M (1974) La formation de l'oocyte d'oiseau. Etude autoradiographique chez la caille japonaise (Coturnix coturnix japonica) pondeuse à l'aide de la leucine tritiée. Arch Biol (Brux) 85:201–233

    Google Scholar 

  • Callebaut M (1975) Bijdrage tot de studie van de oogenesis van de vogels, thesis RUCA, Antwerpen

  • Callebaut M (1978) Effects of centrifugation on living oocytes from adult Japanese quails. Anat Embryol 153:105–113

    Google Scholar 

  • Callebaut M (1979) The avian ovary is an open organ. A study of the lacunar system. Anat Embryol 158:103–119

    Google Scholar 

  • Callebaut M (1980) Persistence of intensely labelled yolk in quail blastoderms after administration of 3H-leucine, during late oogenesis. IRCS Medical Sc 8:660 (1980)

    Google Scholar 

  • Clavert J (1960) Modification de la structure et du comportement physiologique de la paroi du follicule ovarien des oiseaux au cours de la vitellogenèse. Anatom Anzeiger 109:1. Europ Anat Kongr 401–404

    Google Scholar 

  • Davis HW, Sauter RW (1977) Fluorescence of Trypan Blue in frozen-dried Embryos of the Rat. Histochemistry 54:177–189

    Google Scholar 

  • Engels W (1973) Das zeitliche und räumliche Muster der Dottereinlagerung in die Oocyte von Apis mellifica. Z Zellforsch 142:409–430

    Google Scholar 

  • Evans AJ, Perry MM, Gilbert AB (1979) The demonstration of very low density lipoprotein in the basal lamina of the granulosa layer in the hen's ovarian follicle. Biochim Biophys Acta 573:184–195

    Google Scholar 

  • Flickinger RA, Rounds DE (1956) The maternal synthesis of egg yolk proteins as demonstrated by isotopic and serological means. Biochim Biophys Acta 22:38–42

    Google Scholar 

  • Gage SH, Gage SP (1908) Sudan III deposited in the egg and transmitted to chick. Science 28:494–495

    Google Scholar 

  • Goldmann EE (1909) Die äußere und innere Sekretion des gesunden und kranken Organismus im Lichte der “vitalen Färbung” I Beitr Klin Chir 64:192

    Google Scholar 

  • Gonzalez-Santander R (1970) Ultrastructure of the vitellus in blastodise of the chicken. Its evolution in the early stages of development. J Anat 106:111–118

    Google Scholar 

  • Grau CR (1976) Ring structure of avian egg yolk. Poult Sci 55:1418–1422

    Google Scholar 

  • Guraya SS (1957) Histochemical studies of lipids in oocytes. I. Lipids in the oogenesis of Columbia livia. Quart J Microsc Sci 98:407–423

    Google Scholar 

  • Guraya SS (1959) Histochemical studies of lipids in oocytes. IV. Lipids in the oogenesis of Gallus domesticus, Streptopelia senigalensis and decaocto. Res Bull Punjab Univ 10:119–130

    Google Scholar 

  • Guraya SS (1976) Correlative Cytological and Histochemical Studies on the Avian Oogenesis. Z Mikrosk Anat Forsch Leipzig 90:91–150

    Google Scholar 

  • Hamberger A, Hamberger B (1966) Uptake of catecholamines and penetration of trypan blue after blood-brain barrier lesions. Z Zellforsch 70:386–392

    Google Scholar 

  • Hamburger V, Hamilton H (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92

    Google Scholar 

  • Hevesy G, Hahn L (1938) Origin of phosphorus compounds in hen's eggs. Kgl Danske Videnskab Biologiske Meddelesser 14:2, 1–39

    Google Scholar 

  • Homma K, Wilson WO, McFarland LZ (1965) Yolk dye deposition as an index of ovum maturation in Coturnix. Am Zoologist 5:194

    Google Scholar 

  • Korfsmeier KH (1966) Zur Genese des Dottersystems in der Oocyte von Brachydanio rerio. Z Zellforsch mikrosk Anat 71:283–296

    Google Scholar 

  • Koshida Y, Kosin IL (1968) Intranuclear sex dimorphism in the growing feathers of six species of galliformes. Cytologia (Tokyo) 33:230–240

    Google Scholar 

  • Lanot R (1971) Contribution à l'étude du pouvoir tératogène du bleu trypan chez l'embryon de poulet. Arch d'Anat Microscop et Morph Exp 60:49–82

    Google Scholar 

  • Le Douarin N, Barq IL (1969) Sur l'utilisation des cellules de la caille japonaise comme “marqueurs biologiques” en embryologie expérimentale. C R Acad Sci Paris 269:1543–1546

    Google Scholar 

  • Marza VD, Marza RV (1935) The formation of the hen's eggs. I-IV Quart J microsc Sci 78:134–189

    Google Scholar 

  • McNally EH (1943) The origin and structure of the vitelline membrane of the domestic fowl's egg. Poult Sci 22:40

    Google Scholar 

  • Pander C (1817) Historiam metamorphoseos quam ovum incubatum prioribus quinque diebus subit. Wirceburgi

  • Perry MM, Gilbert AB (1979) Yolk transport in the avian follicle of the hen (Gallus domesticus): lipoprotein-like particles at the periphery of the oocyte in the rapid growth phase. J Cell Sci 39:257–272

    Google Scholar 

  • Perry MM, Gilbert AB, Evans AJ (1978a) Electron microscope observations on the ovarian follicle of the domestic fowl during the rapid growth phase. J Anat 125:481–497

    Google Scholar 

  • Perry MM, Gilbert AB, Evans AJ (1978b) The structure of the germinal disc region of the hen's ovarian follicle during the rapid growth phase. J Anat 127:379–392

    Google Scholar 

  • Ramamurty PS (1964) On the contribution of the follicle epithelium to the deposition of yolk in the oocyte of Panorpa communis. Expl Cell Res 33:601–605

    Google Scholar 

  • Riddle O (1911) On the formation, significance and chemistry of the white and yellow yolk of ova. J Morphol 22:455–491

    Google Scholar 

  • Robinson G (1973) The oocytes as major competitors for radioiodide in laying Japanese quail. Gen Comp Endocrinol 21:123–128

    Google Scholar 

  • Romeis B (1948) Mikroskopische Technik. 15. Aufl Leibnitz, München

    Google Scholar 

  • Rothwell B, Solomon SE (1977) The ultrastructure of the follicle wall of the domestic fowl during the phase of rapid growth. Br Poult Sci 18:605–610

    Google Scholar 

  • Smith AH (1959) Follicular permeability and yolk formation. Poult Science 38:1437–1446

    Google Scholar 

  • Smith AH, Winget CM, Blackard JR (1954) The transfer of phosphorus to the hen's egg, under controlled environment, as traced with radiophosphorus. Poult Science 33:908–919

    Google Scholar 

  • Steinwall O, Klatzo I (1966) Selective vulnerability of the blood-brain barrier in chemically induced lesions. J Neuropath Exp Neurol 25:542–559

    Google Scholar 

  • Sturkie PD (1954) Avian Physiology. Comstock, Ithaca

  • Thilander H (1964) Histologic technique for tissues stained with trypan blue. Anat Anz 115:89–107

    Google Scholar 

  • Vakaet L (1962) Morfologische en experimentele studie over de pregastrulatie en gastrulatie der vogelkiem. Arscia Uitgaven, Brussel

    Google Scholar 

  • Van Durme M (1914) Nouvelles recherches sur la vitellogénèse des oeufs aux stades d'accroissement, de maturation, de fécondation et du début de la segmentation. Arch Biol Paris 29:71–200

    Google Scholar 

  • Wallace RA, Ho T (1972) Protein incorporation by isolated amphibian Oocytes. II. A survey of inhibitors. J Exp Zool 181:303–318

    Google Scholar 

  • Warren DC, Conrad RM (1939) Growth of the hen's ovum. J Agric Res 58:875–893

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Anatomy and Embryology, R.U.C.A., Groenenborgerlaan 171, B-2020, Antwerpen, (Belgium)

    Marc Callebaut & Lucien Vakaet

Authors
  1. Marc Callebaut
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fernand Harrisson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lucien Vakaet
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Callebaut, M., Harrisson, F. & Vakaet, L. Peripheral avian yolk assemblage and its persistence in the blastoderm, studied by trypan blue-induced fluorescence. Anat Embryol 163, 55–69 (1981). https://doi.org/10.1007/BF00315770

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00315770

Key words

Search

Navigation