Abstract
The hypothalamic suprachiasmatic nucleus is centrally involved in generation of several circadian rhythms. Neurons of the mammalian suprachiasmatic nucleus express a number of neuropeptides including vasopressin. The suprachiasmatic nucleus of the mink (Mustela vison) is easily distinguished from neighbouring hypothalamic areas and the underlying optic chiasm as a small nucleus containing densely packed parvocellular neurons. A dorsal and ventral subdivision were clearly recognized within the midportion and caudal part of the nuclcus. Using immunohistochemistry, we have identified vasopressin-, neurophysin-, and vasoactive intestinal peptide-immunoreactive neuronal elements in the hypothalamus of the mink. Vasoactive intestinal peptide-immunoreactive neurons can be observed in the ventral aspect of the suprachiasmatic nucleus, but to our surprise, no vasopressin immunoreactive perikarya are found within the suprachiasmatic nucleus, this absence being independent of the experienced annual cycle. The hypothalamic paraventricular and supraoptic nuclei contain large numbers of vasopressin-, neurophysin-and vasoactive intestinal peptide-immunoreactive magnocellular neurons with extensive projections towards the infundibulum and neurohypophysis. A comparative analysis of the distribution of vasopressin-immunoreactive elements in a number of conventional laboratory animals has demonstrated that, in contrast to the rat, golden hamster and Mongolian gerbil, neither vasopressin-containing perikarya in the suprachiasmatic nucleus nor fine calibered immunoreactive fibres entering the adjacent subparaventricular zone are present in the mink. The mink is a photodependent seasonal breeder, and thus vasopressin-immunoreactive neurons in the suprachiasmatic nuclei may not be essential for the photoperiodic regulation of reproduction and seasonal events experienced by this species.
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References
Card JP, Brecha N, Karten HJ, Moore RY (1981) Immunocytochemical localization of vasoactive intestinal polypeptide-containing cells and processes in the suprachiasmatic nucleus of the rat: light and electron microscopic analysis. J Neurosci 1:1289–1303
Duncan MJ, goldman BD (1984) Hormonal regulation of the annual pelage colour cycle in the Djungarian hamster, Phodopus sungorus. II. Role of prolactin. J Exp Zool 230:97–103
Earnest DJ, Sladek CD (1986) Circadian rhythms of vasopressin release from indivdual rat suprachiasmatic explants in vitro. Brain Res 382:129–133
Eckland DJA, Todd K, Lightman SL (1987) Immunoreactive vasopressin and oxytocin in hypothalamo-hypophysial protal blood of the Brattleboro and Long-Evans rat: Effect of adrenalectomy and dexamethasone. J Endocrinol 117:27–34
Gillette MU, Reppert SM (1987) The hypothalamic suprachiasmatic nuclei: circadian patterns of vasopressin secretion and neuronal activity in vitro. Brain Res Bull 19:135–139
Glass JD, Knotts LK (1987) A brain site for the antigonadal action of melatonin in the white-footed mouse (Peromyscus leucopus): involvement of the immunoreactive GnRH neuronal system. Neuroendocrinology 46:48–55
Groblewski TA, Nunez AA, Gold RM (1981) Circadian rhythms in vasopressin deficient rats. Brain Res Bull 6:125–130
Hastings MH (1991) Neuroendocrine rhythms. Pharmacol Ther 50:35–71
Hornemann EMD, Buijs RM (1982) Vasopressin fiber pathways in the rat brain following suprachiasmatic nucleus lesioning. Brain Res 243:235–241
Inouye ST, Kawamura H (1979) Persistence of circadian rhythmicity in a mammalian hypothalamic “island” containing the suprachiasmatic nucleus. Proc Natl Acad Sci USA 76:5962–5966
Johnson RF, Morin LP, Moore RY (1988) Retinohypothalamic projections in the hamster and rat demonstrated using cholera toxin. Brain Res 462:301–312
Karsch FJ, Bittman EL, Foster DL, Goodman RL, Legan SJ, Robinson JE (1984) Neuroendocrine basis of seasonal reproduction. Recent Prog Hormone Res 40:185–232
Levine JD, Weiss ML, Rosenwasser AM, Miselis RR (1991) Retinohypothalamic tract in the female albino rat: a study using horseradish peroxidase conjugated to cholera toxin. J Comp Neurol 306:344–360
Martinet L, Allain D (1985) Role of the pineal gland in the photoperiodic control of reproductive and non-reproductive functions in mink (Mustela vison). In: Evered D, Clark S (eds) Photoperiodism, melatonin and the pineal. CIBA foundation Symposium 117, Pitman, London, pp 170–187
Martinet L, Allain D, Weiner C (1984) The role of prolactin in photoperiodic control of moulting in the mink. J Endocrinol 103:9–15
Meijer JH, Rietveld WJ (1989) Neurophysiology of suprachiasmatic circadian pacemaker in rodents. Physiol Rev 69:671–707
Mikkelsen JD (1990) Projections from the lateral geniculate nucleus to the hypothalamus of the Mongolian gerbil (Meriones unguiculatus): an anterograde and retrograde tracing study. J Comp Neurol 299:493–508
Mikkelsen JD, Larsen PJ, O'Hare MMT, Wiegand SJ (1991) Gastrin releasing peptide (GRP) in the rat suprachiasmatic nucleus. An immunohistochemical, chromatographic and radioimmunological study. Neuroscience 40:55–66
Murakami N Takamure M, Takahashi K, Utunomiya K, Kuroda H, Etoh T (1991) Longterm cultured neurons from rat suprachiasmatic nucleus retain the capacity fro circadian oscillation of vasopressin release. Brain Res 545:347–350
Pickard GE (1985) Bifurcation axons of retinal ganglion cells terminate in the hypothalamic suprachiasmatic nucleus and the intergeniculate leaflet of the thalamus. Neurosci Lett 55:211–217
Pickard GE, Silverman AJ (1979) Effects of photoperiod on hypothalamic luteinizing hormone releasing hormone in the male hamster. J Endocrinol 83:421–428
Ralph MR, Foster RG, Davis FC, Menaker M (1990) Transplanted suprachiasmatic nucleus determines circadian period. Science 247:975–978
Reppert SM, Schwartz WJ, Uhl GR (1987) Arginine vasopressin: A novel peptide rhythm in cerebrospinal fluid. Trends Neurosci 10:76–80
Reppert SM, Weaver DR, Rivkees SA, Stopa EG (1988) Putative melatonin receptors in a human biological clock. Science 242:78–81
Rusak B, Zucker I (1979) Neural regulation of circadian rhythms. Physiol Behav 59:449–526
Schwartz WJ, Reppert SM (1985) Neural regulation of circadian vasopressin rhythm in cerebrospinal fluid: a preeminent role for the suprachiasmatic nucleus. J Neurosci 5:2771–2778
Schwartz WJ, Davidson LC, Smith CB (1980) In vivo metabolic activity of putative circadian oscillator. The rat suprachiasmatic nucleus. J Comp Neurol 189:157–167
Shibata S, Oomura Y, Hattori K, Kita H (1984) Responses of suprachiasmatic nucleus neurons to optic nerve stimulation in rat hypothalamic slice preparations. Brain Res 302:82–89
Sofroniew MV, Weindl A (1980) Identification of parvocellular vasopressin and neurophysin neurons in the suprachiasmatic nucleus of a variety of mammals including primates. J Comp Neurol 193:659–675
Stephan FK, Zucker I (1972) Circadian rhythms in drinking behavior and locomotor activity of rats are eliminated by hypothalamic lesions. Proc Natl Acad Sci USA 69:1583–1586
Tamarkin L, Baird CJ, Almeida OFX (1985) Melatonin: a coordinating signal for mammalian reproduction? Science 227:714–720
Uhl GR, Reppert SM (1986) Suprachiasmatic nucleus vasopressin messenger RNA: circadian variation in normal and Brattleboro rats. Science 232:390–393
Van den Pol AN (1980) The hypothalmic suprachiasmatic nucleus of the rat: instrinsic anatomy. J Comp Neurol 191:661–702
Van den Pol AN, Tsujimoto KL (1985) Neurotransmitters of the hypothalamic suprachiasmatic nucleus: Immunocytochemical analysis of 25 neuronal antigens. Neuroscience 15:1049–1086
Vandesande F, Dierrickx K, De May J (1975) Identification of the vasopressin-neurophysin producing neurons of the suprachiasmatic nuclei. Cell Tissue Res 156:377–380
Vaněček J (1988) Melatonin binding sites. J Neurochem 51:1436–1440
Watts AG, Swanson LW, Sanchez-Watts G (1987) Efferent projections of the suprachiasmatic nucleus. I. Studies using anterograde transport of Phaseolus vulgaris leucoagglutinin in the rat. J Comp Neurol 258:204–229
Weaver DR, Reppert SM (1990) Melatonin receptors are present in the ferret pars tuberalis and pars distalis, but not in brain. Endocrinology 127:2607–2609
Weaver DR, Rivkees SA, Reppert SM (1989) Localization and characterization of melatonin receptors in rodent brain by in vitro autoradiography. J Neurosci 9:2581–2590
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Larsen, P.J., Mikkelsen, J.D. The suprachiasmatic nucleus of the mink (Mustela vison): apparent absence of vasopressin-immunoreactive neurons. Cell Tissue Res 273, 239–247 (1993). https://doi.org/10.1007/BF00312825
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DOI: https://doi.org/10.1007/BF00312825