Summary
Mutations in the RAD50 gene of Saccharomyces cerevisiae have been shown to reduce double strand break repair, meiotic recombination, and radiation-inducible mitotic recombination. Several different point mutations (including ochre and amber alleles) have been previously examined for effects on spontaneous mitotic recombination and did not reduce the frequency of recombination. Instead, the rad50 mutations conferred a moderate hyper-rec phenotype. This paper examines a deletion/interruption allele of RAD50 that removes 998 of 1312 amino acids and adds 1.1 kb of foreign DNA. The results clearly indicate that spontaneous mitotic recombination can occur in the absence of RAD50; in fact, the frequency of recombination is elevated over the wild-type cell. One possible interpretation of these observations is that the initiating lesion in spontaneous recombination events in mitosis might not be a double strand break.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alani E, Subbiah S, Kleckner N (1989) Genetics 122:47–57
Chow T, Resnick M (1988) The identification of a deoxyribonuclease controlled by the RAD52 gene of Saccharomyces cerevisiae. In: Friedberg E, Bridges B (eds) Cellular responses to DNA damage. Liss, New York, pp 447–455
Esposito MS, Wagstaff JE (1981) The molecular biology of the yeast Saccharomyces. I. Life cycle and inheritance. In: Strathern JN, Jones EW, Broach JR (eds) Mechanisms of mitotic recombination. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York pp 341–370
Farnet C, Padmore R, Cao L, Raymond W, Alani E, Kleckner N (1988) UCLA Symp Mol Cell Biol 83:201–215
Friedberg EC (1988) Microbiol Rev 52:70–102
Game JC (1983) Radiation-sensitive mutants and repair in yeast. In: Spencer JFT, Spencer DM, Smith ARW (eds) Yeast genetics. Fundamental and applied aspects. Springer, Berlin Heidelberg New York, pp 109–137
Game JC, Zamb TJ, Braun RJ, Resnick MA, Roth RM (1980) Genetics 94:51–68
Hoekstra MF, Malone RE (1985) Mol Cell Biol 5:610–618
Holliday R (1964) Genet Res (Cambridge) 5:282–304
Malone RE (1983) Mol Gen Genet 189:405–412
Malone RE, Esposito RE (1980) Proc Natl Acad Sci USA 77:503–507
Malone RE, Esposito RE (1981) Mol Cell Biol 1:891–901
Malone RE, Hoekstra MF (1984) Genetics 107:33–48
Malone RE, Hyman D (1983) Curr Genet 7:439–447
Malone RE, Jordan KB, Wardmam W (1985) Curr Genet 9:453–461
Malone RE, Montelone B, Edwards C, Carney K, Hoekstra MF (1988) Curr Genet 14:211–223
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Meselson M, Radding C (1975) Proc Natl Acad Sci USA 72:358–361
Montelone BA, Hoekstra MF, Malone RE (1988) Genetics 119:289–301
Orr-Weaver TL, Szostak JW, Rothstein RJ (1981) Proc Natl Acad Sci USA 78:6354–6358
Prakash S, Prakash L, Burke W, Montelone BA (1980) Genetics 94:31–50
Radding C (1982) Annu Rev Genet 16:405–438
Resnick M, Nitiss J, Edwards C, Malone R (1986) Genetics 104:603–618
Resnick MA (1976) J Theor Biol 59:97–112
Resnick MA, Martin P (1976) Mol Gen Genet 143:119–129
Roman H (1956) C R Trav Lab Carlsberg 26:299–304
Szostak JW, Orr-Weaver TL, Rothstein RJ, Stahl F (1983) Cell 33:24–35
Thaler DS, Stahl FW (1988) Annu Rev Genet 22:169–197
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Malone, R.E., Ward, T., Lin, S. et al. The RAD50 gene, a member of the double strand break repair epistasis group, is not required for spontaneous mitotic recombination in yeast. Curr Genet 18, 111–116 (1990). https://doi.org/10.1007/BF00312598
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00312598