Abstract
Manual and automatic determinations of Sarin with a fluoride selective electrode after alkaline hydrolysis are presented. Comparison with a classic colorimetric method shows excellent agreement between the two methods.
Enzymatic hydrolysis (acetylcholinesterase) measured with an enzyme-electrode, yields no results: the assumption that Sarin loses F− upon reaction with cholinesterase is proved to be incorrect.
Zusammenfassung
Ein manuelles und ein automatisches Verfahren zur Bestimmung von Sarin nach alkalischer Hydrolyse durch Anwendung einer fluoridselektiven Elektrode werden beschrieben. Der Vergleich mit einem klassischen colorimetrischen Verfahren zeigt hohe Übereinstimmung der Resultate beider Methoden.
Die enzymatische Hydrolyse (Acetylcholinesterase) unter Anwendung einer Enzymelektrode, gibt keine Resultate: die Annahme, daß Sarin bei der Reaktion mit Cholinesterase F− verliert, ist nicht richtig.
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De Clercq, H., Mertens, J. & Massart, D.L. Fluoride selective electrode procedure for fluorated organophosphate cholinesterase inhibitors. Arch Toxicol 31, 185–191 (1973). https://doi.org/10.1007/BF00310397
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DOI: https://doi.org/10.1007/BF00310397