Summary
Fetuin, α2HS-glycoprotein (α2HS), α-fetoprotein (AFP) and albumin have been shown to be present in some regions of the neocortex in two early stages of development of the cow brain using PAP immunocytochemistry. In the pre-cortical plate stage fibres of the primordial plexiform layer stained positively for fetuin. No staining was seen for albumin but plasma and cerebrospinal fluid (CSF) were positive for α2HS and AFP. In the early cortical plate stage the strongest fetuin positive staining was seen in the earliest formed cells of the plate. α2HS staining was much less intense but similar in distribution. The possible role of fetuin, or related glycoproteins, in cortical plate differentiation is discussed. Staining for AFP and for albumin was seen mainly in the ventricular zone and marginal zone fibres, and had a similar distribution and intensity for both proteins. Plasma and CSF stained for all four proteins. Tests showed some cross-reactivity between fetuin and anti-α2HS and, to a much lesser extent, between antisera to AFP and albumin and antigens denatured by fixation.
Similar content being viewed by others
References
Benno RH, Williams TH (1978) Evidence for the intracellular localization of alpha-fetoprotein in the developing rat brain. Brain Res 142:182–186
Bergroth V, Reitamo S, Konttinen YT, Lalla M (1980) Sensitivity and non-specific staining of various immunoperoxidase techniques. Histochem 68:17–22
Bullock GR, Petrusz P (1982) Techniques in immunocytochemistry. Vol 1, Academic Press
Cavanagh ME, Møllgård K (1985) An immunocytochemical study of the distribution of some plasma proteins within the developing forebrain of the pig with special reference to the neocortex. Dev Brain Res 17:183–194
Cavanagh ME, Cornelis MEP, Dziegielewska KM, Luft AJ, Lorscheider FL, Saunders NR (1982) Proteins in cerebrospinal fluid and plasma of fetal pigs during development. Dev Neurosci 5:492–502
Cavanagh ME, Cornelis MEP, Dziegielewksa KM, Evans CAN, Lorscheider FL, Møllgård K, Reynolds ML, Saunders NR (1983) Comparison of proteins in csf of forebrain and hindbrain during early development of fetal sheep. Dev Brain Res 11:159–167
Childs GV (1983) The use of multiple methods to validate immunocytochemical stains. J Histochem Cytochem 31:168–176
Christie D, Dziegielewska KM, Le Gros G, Møllgård K, Renwick AGC, Reynolds ML, Saunders NR (1986) α2HS Glycoprotein, the human equivalent of the fetal protein fetuin. Physiological Society, Cambridge Meeting
Dziegielewska KM, Evans CAN, Lai PCW, Lorscheider FL, Malinowska DH, Møllgård K, Saunders NR (1981) Proteins in cerebrospinal fluid and plasma of fetal rats during development. Dev Biol 83:193–200
Dziegielewska KM, Hinds LA, Sarantis MEP, Saunders NR, Tyndale-Biscoe CH (1986a) Proteins in cerebrospinal fluid and plasma of the pouch young tammar wallaby (Macropus eugenii) during development. Comp. Biochem. Physiol. 83B:561–567
Dziegielewska KM, Møllgård K, Reynolds, ML, Saunders NR (1986b) A fetuin-related protein (α2HS) in human embryonic and fetal development. Cell and Tissue Res (accepted for publication)
Dziegielewska KM, Saunders NR, Schejter EJ, Zakut H, Zevin-Sonkin D, Zisling R, Soreq H (1986c) Synthesis of plasma proteins in fetal, adult and neoplastic human brain tissue. Dev Biol 155:93–104
Eiferman FA, Young PR, Scott RW, Tilghman SM (1981) Intragenic amplification and divergence in the mouse α-fetoprotein gene. Nature 294:713–718
Grube D (1980) Immunoreactivites of gastrin (G) cells 2. Non-specific binding of immunoglobulins to G-cells by ionic interactions. Histochemistry 66:149–167
Grube D, Weber E (1980) Immunoreactivities of gastrin (G) cells 1. Dilution-dependent staining of G-cells by antisera and nonimmune sera. Histochemistry 65:223–237
Hutson JC, Childs GV, Garnder PF (1979) Considerations for establishing the validity of immunocytological studies. J Histochem Cytochem 27:1201–1203
Janzen RG, Mably, ER, Tamaoki T, Church RB, Lorscheider FL (1982) Synthesis of alpha-fetoprotein by the preimplantation and post implantation bovine embryo. J Reprod Fertil 65:105–110
Law SW, Dugaiezyk A (1981) Homology between the primary structure of α-fetoprotein, deduced from a complete cDNA sequence, and serum albumin. Nature 291:201–205
Marin-Padilla M (1983) Structural organization of the human cerebral cortex prior to the appearance of the cortical plate. Anat Embryol 168:21–40
Marin-Padilla M, Marin-Padilla TM (1982) Origen, prenatal development and structural organization of layer 1 of the human cerebral (motor) cortex. Anat Embryol 164:161–206
Møllgård K, Jacobsen M (1984) Immunohistochemical identification of some plasma proteins in human embryonic and fetal forebrain with particular reference to the development of the neocortex. Dev Brain Res 13:49–63
Møllgård K, Reynolds ML, Jacobsen M, Dziegielewska KM, Saunders NR (1984) Differential immunocytochemical staining for fetuin and transferrin in the developing cortical plate. J Neurocytol 13:497–502
Moro R, Uriel J (1981) Early localization of alpha-fetoprotein in the development nervous system of the chicken. Oncodev Biol Med 2:391–398
Moro R, Fieditz W, Esteves A, Grunberg J, Uriel J (1984) In vivo uptake of heterologous alpha-fetoprotein and serum albumin by ependymal cells of developing chick embryos. Int J Dev Neurosci 2:143–148
Pedersen KO (1944) Fetuin, a new globulin isolated from serum. Nature 154:575
Petrusz P, Ordronneau P, Finley JCM (1980) Criteria of reliability for light microscopic immunocytochemical staining. Histochem J 12:333–348
Pineiro A, Calvo M, Iguaz F, Lampreave F, Naval J (1982) Characterization, origen and evolution of α-fetoprotein and albumin in postnatal rat brain. Int J Biochem 14:817–823
Readler E, Raedler A, Feldhaus S (1980) Dynamical aspects of neocortical histogenesis in the rat. Anat. Embryol 158:253–269
Reynolds ML, Møllgård K (1985) The distribution of plasma proteins in the neocortex and early allocortex of the developing sheep brain. Anat Embryol 171:41–60
Reynolds ML, Cavanagh ME, Dziegielewska KM, Hinds LA, Saunders NR, Tyndale-Biscoe, CH (1985) Postnatal development of the telencephalon of the tammar wallaby (Macropus eugenii). An accessible model of neocortical differentiation. Anat Embryol 173:81–94
Ruoslahti E, Engvall E (1976) Immunological cross-reaction between alpha-fetoprotein and albumin. Proc Natl Acad Sci USA 73:4641–4644
Sarantis MEP, Saunders NR (1986) An immunocytochemical demonstration of α2HS-glycoprotein in the developing neocortex of the rat. Neurosci Lett 65:346–350
Saunders NR (1985) Plasma proteins and cerebral cortical development. In: Development, Organisation and Processing in Somatosensory Pathways. Alan R Liss Inc, pp 79–86
Sell S, Becker FF (1978) Alpha-fetoprotein. J Natl Cancer Inst 60:19–26
Sell S, Longley MA, Boulter J (1985) α-Fetoprotein and albumin gene expression in brain and other tissues of fetal and adult rats. Dev Brain Res 22:49–53
Sidman RC, Rakic P (1973) Neuronal migration with special reference to developing human brain: a review. Brain Res 62:1–35
Smith KM, Lai PCW, Robertson HA, Church RB, Lorscheider FL (1979). Distribution of alpha-fetoprotein in fetal plasma, allantoic fluid, amniotic fluid and maternal plasma of cows. J Reprod Fertil 57:235–238
Sternberger LA (1979) Immunocytochemistry 2nd Edition. John Wiley and Sons, New York, pp 89–92
Suzuki Y, Kamata Y, Tanaka N, Kaneda H, Hirai H (1984) Immunohistochemical study on alpha-fetoprotein in rats during fetal and postnatal life. Protides Biol Fluids 31:391–394
Toran-Allerand CD (1980) Co-existence of alpha-fetoprotein, albumin and transferrin immunoreactivity in neurons of the developing mouse brain. Nature 288:733–735
Uriel J, Trojan J, Dubouch P, Pineiro A (1982) Intracellular alphafetoprotein and albumin in the developing nervous system of the baboon. Pathol Biol 30:79–83
Vandesande F (1979) A critical review of immunocytochemical methods for light microscopy. J Neurosci Methods 1:3–23
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reynolds, M.L., Sarantis, M.E.P., Lorscheider, F.L. et al. Fetuin as a marker of cortical plate cells in the fetal cow neocortex: a comparison of the distribution of fetuin, α2HS-glycoprotein, α-fetoprotein and albumin during early development. Anat Embryol 175, 355–363 (1987). https://doi.org/10.1007/BF00309848
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00309848